Highly selective and sensitive nucleic acid detection based on polysaccharide-functionalized silver nanoparticles

Jing Kun Yan, Hai Le Ma, Pan Fu Cai, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

10 Citations (Scopus)


Polysaccharide-functionalized silver nanoparticles (Oc-AgNPs) with a mean diameter of 15 nm were utilized as a novel and effective fluorescence-sensing platform for nucleic acid detection. Tests on the oligonucleotide sequences associated with the human immunodeficiency virus as a model system showed that the Oc-AgNPs effectively absorbed and quenched dye-labeled single-stranded DNA through strong hydrogen bonding interactions and slight electrostatic attractive interactions. The proposed system efficiently differentiated between complementary and mismatched nucleic acid sequences with high selectivity and good reproducibility at room temperature.
Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Publication statusPublished - 5 Jan 2015


  • Fluorescent sensing platform
  • Nucleic acid detection
  • Polysaccharide
  • Silver nanoparticles

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy

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