Highly selective and sensitive nucleic acid detection based on polysaccharide-functionalized silver nanoparticles

Jing Kun Yan; Hai Le Ma; Pan Fu Cai; Jianyong Wu

doi:10.1016/j.saa.2014.06.079

Highly selective and sensitive nucleic acid detection based on polysaccharide-functionalized silver nanoparticles

Jing Kun Yan, Hai Le Ma, Pan Fu Cai, Jianyong Wu

Research output: Journal article publication › Journal article › Academic research › peer-review

13 Citations (Scopus)

Abstract

Polysaccharide-functionalized silver nanoparticles (Oc-AgNPs) with a mean diameter of 15 nm were utilized as a novel and effective fluorescence-sensing platform for nucleic acid detection. Tests on the oligonucleotide sequences associated with the human immunodeficiency virus as a model system showed that the Oc-AgNPs effectively absorbed and quenched dye-labeled single-stranded DNA through strong hydrogen bonding interactions and slight electrostatic attractive interactions. The proposed system efficiently differentiated between complementary and mismatched nucleic acid sequences with high selectivity and good reproducibility at room temperature.

Original language	English
Pages (from-to)	17-21
Number of pages	5
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	134
DOIs	https://doi.org/10.1016/j.saa.2014.06.079
Publication status	Published - 5 Jan 2015

Keywords

Fluorescent sensing platform
Nucleic acid detection
Polysaccharide
Silver nanoparticles

ASJC Scopus subject areas

Analytical Chemistry
Atomic and Molecular Physics, and Optics
Instrumentation
Spectroscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.saa.2014.06.079

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title = "Highly selective and sensitive nucleic acid detection based on polysaccharide-functionalized silver nanoparticles",

abstract = "Polysaccharide-functionalized silver nanoparticles (Oc-AgNPs) with a mean diameter of 15 nm were utilized as a novel and effective fluorescence-sensing platform for nucleic acid detection. Tests on the oligonucleotide sequences associated with the human immunodeficiency virus as a model system showed that the Oc-AgNPs effectively absorbed and quenched dye-labeled single-stranded DNA through strong hydrogen bonding interactions and slight electrostatic attractive interactions. The proposed system efficiently differentiated between complementary and mismatched nucleic acid sequences with high selectivity and good reproducibility at room temperature.",

keywords = "Fluorescent sensing platform, Nucleic acid detection, Polysaccharide, Silver nanoparticles",

author = "Yan, {Jing Kun} and Ma, {Hai Le} and Cai, {Pan Fu} and Jianyong Wu",

year = "2015",

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day = "5",

doi = "10.1016/j.saa.2014.06.079",

language = "English",

volume = "134",

pages = "17--21",

journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",

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Highly selective and sensitive nucleic acid detection based on polysaccharide-functionalized silver nanoparticles. / Yan, Jing Kun; Ma, Hai Le; Cai, Pan Fu et al.
In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 134, 05.01.2015, p. 17-21.

Research output: Journal article publication › Journal article › Academic research › peer-review

TY - JOUR

T1 - Highly selective and sensitive nucleic acid detection based on polysaccharide-functionalized silver nanoparticles

AU - Yan, Jing Kun

AU - Ma, Hai Le

AU - Cai, Pan Fu

AU - Wu, Jianyong

PY - 2015/1/5

Y1 - 2015/1/5

N2 - Polysaccharide-functionalized silver nanoparticles (Oc-AgNPs) with a mean diameter of 15 nm were utilized as a novel and effective fluorescence-sensing platform for nucleic acid detection. Tests on the oligonucleotide sequences associated with the human immunodeficiency virus as a model system showed that the Oc-AgNPs effectively absorbed and quenched dye-labeled single-stranded DNA through strong hydrogen bonding interactions and slight electrostatic attractive interactions. The proposed system efficiently differentiated between complementary and mismatched nucleic acid sequences with high selectivity and good reproducibility at room temperature.

AB - Polysaccharide-functionalized silver nanoparticles (Oc-AgNPs) with a mean diameter of 15 nm were utilized as a novel and effective fluorescence-sensing platform for nucleic acid detection. Tests on the oligonucleotide sequences associated with the human immunodeficiency virus as a model system showed that the Oc-AgNPs effectively absorbed and quenched dye-labeled single-stranded DNA through strong hydrogen bonding interactions and slight electrostatic attractive interactions. The proposed system efficiently differentiated between complementary and mismatched nucleic acid sequences with high selectivity and good reproducibility at room temperature.

KW - Fluorescent sensing platform

KW - Nucleic acid detection

KW - Polysaccharide

KW - Silver nanoparticles

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U2 - 10.1016/j.saa.2014.06.079

DO - 10.1016/j.saa.2014.06.079

M3 - Journal article

C2 - 24995414

SN - 1386-1425

VL - 134

SP - 17

EP - 21

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

ER -

Highly selective and sensitive nucleic acid detection based on polysaccharide-functionalized silver nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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