High Resolution Quantitative Angle-Scanning Widefield Surface Plasmon Microscopy

Han Min Tan, Suejit Pechprasarn, Jing Zhang, Mark C. Pitter, Michael Geoffrey Somekh

Research output: Journal article publicationJournal articleAcademic researchpeer-review

17 Citations (Scopus)

Abstract

We describe the construction of a prismless widefield surface plasmon microscope; this has been applied to imaging of the interactions of protein and antibodies in aqueous media. The illumination angle of spatially incoherent diffuse laser illumination was controlled with an amplitude spatial light modulator placed in a conjugate back focal plane to allow dynamic control of the illumination angle. Quantitative surface plasmon microscopy images with high spatial resolution were acquired by post-processing a series of images obtained as a function of illumination angle. Experimental results are presented showing spatially and temporally resolved binding of a protein to a ligand. We also show theoretical results calculated by vector diffraction theory that accurately predict the response of the microscope on a spatially varying sample thus allowing proper quantification and interpretation of the experimental results.
Original languageEnglish
Article number20195
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 1 Feb 2016

ASJC Scopus subject areas

  • General

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