Hepatic PRMT1 ameliorates diet-induced hepatic steatosis via induction of PGC1α

Lu Xu, Zhe Huang, Tak Ho Lo, Ranyao Yang, Tsz Hang Jimmy Lee, Xingqun Yan, Dewei Ye, Aimin Xu, Chi Ming Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

Rationale: Over-nutrition will lead to overexpression of PRMT1 but protein hypomethylation is observed in the liver of obese subjects. The dynamic alteration of the expression and methyltransferase activity of PRMT1 in the progression of fatty liver diseases remains elusive.

Methods: We used recombinant adeno-associated virus-mediated gene delivery system to manipulate the hepatic PRMT1 expression level in diet-induced obese mice to investigate the role of PRMT1 in hepatic steatosis. We further utilized a cohort of obese humans with biopsy-proven nonalcoholic fatty liver disease to support our observations in mouse model.

Results: We demonstrated that knockdown of PRMT1 promoted steatosis development in liver of high-fat diet (HFD) fed mice. Over-expression of wild-type PRMT1, but not methyltransferase-defective mutant PRMT1G80R, could alleviate diet-induced hepatic steatosis. The observation is conserved in the specimens of obese humans with biopsy-proven nonalcoholic fatty liver disease. Mechanistically, methyltransferase activity of PRMT1 was required to induce PGC-1α mRNA expression via recruitment of HNF-4α to the promoter of PGC-1α, and hence attenuated HFD-induced hepatic steatosis by enhancing PGC-1α-mediated fatty acid oxidation.

Conclusions: Our results identify that activation of the PRMT1/HNF-4α/PGC-1α signaling is a potential therapeutic strategy for combating non-alcoholic fatty liver disease of obese subjects.
Original languageEnglish
JournalTheranostics
Publication statusPublished - 2022

Keywords

  • Non-alcoholic fatty liver disease (NAFLD)
  • Diet-induced hepatic steatosis
  • PRMT1
  • PGC-1α
  • HNF-4α

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