Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J. Klionsky; Kotb Abdelmohsen; Akihisa Abe; Md Joynal Abedin; Hagai Abeliovich; Abraham Acevedo Arozena; Hiroaki Adachi; Christopher M. Adams; Peter D. Adams; Khosrow Adeli; Peter J. Adhihetty; Sharon G. Adler; Galila Agam; Rajesh Agarwal; Manish K. Aghi; Maria Agnello; Patrizia Agostinis; Patricia V. Aguilar; Julio Aguirre-Ghiso; Edoardo M. Airoldi; Slimane Ait-Si-Ali; Takahiko Akematsu; Emmanuel T. Akporiaye; Mohamed Al-Rubeai; Guillermo M. Albaiceta; Chris Albanese; Diego Albani; Matthew L. Albert; Jesus Aldudo; Hana Algül; Mehrdad Alirezaei; Iraide Alloza; Alexandru Almasan; Maylin Almonte-Beceril; Emad S. Alnemri; Covadonga Alonso; Nihal Altan-Bonnet; Dario C. Altieri; Silvia Alvarez; Lydia Alvarez-Erviti; Sandro Alves; Giuseppina Amadoro; Atsuo Amano; Consuelo Amantini; Santiago Ambrosio; Ivano Amelio; Amal O. Amer; Mohamed Amessou; Angelika Amon; Zhenyi An; Frank A. Anania; Stig U. Andersen; Usha P. Andley; Catherine K. Andreadi; Nathalie Andrieu-Abadie; Alberto Anel; David K. Ann; Shailendra Anoopkumar-Dukie; Manuela Antonioli; Hiroshi Aoki; Nadezda Apostolova; Saveria Aquila; Katia Aquilano; Koichi Araki; Eli Arama; Agustin Aranda; Jun Araya; Alexandre Arcaro; Esperanza Arias; Hirokazu Arimoto; Aileen R. Ariosa; Jane L. Armstrong; Thierry Arnould; Ivica Arsov; Katsuhiko Asanuma; Valerie Askanas; Eric Asselin; Ryuichiro Atarashi; Sally S. Atherton; Julie D. Atkin; Laura D. Attardi; Patrick Auberger; Georg Auburger; Laure Aurelian; Riccardo Autelli; Laura Avagliano; Maria Laura Avantaggiati; Limor Avrahami; Neelam Azad; Suresh Awale; Tiziana Bachetti; Jonathan M. Backer; Dong Hun Bae; Jae Sung Bae

doi:10.1080/15548627.2015.1100356

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J. Klionsky, Kotb Abdelmohsen, Akihisa Abe, Md Joynal Abedin, Hagai Abeliovich, Abraham Acevedo Arozena, Hiroaki Adachi, Christopher M. Adams, Peter D. Adams, Khosrow Adeli, Peter J. Adhihetty, Sharon G. Adler, Galila Agam, Rajesh Agarwal, Manish K. Aghi, Maria Agnello, Patrizia Agostinis, Patricia V. Aguilar, Julio Aguirre-Ghiso, Edoardo M. AiroldiSlimane Ait-Si-Ali, Takahiko Akematsu, Emmanuel T. Akporiaye, Mohamed Al-Rubeai, Guillermo M. Albaiceta, Chris Albanese, Diego Albani, Matthew L. Albert, Jesus Aldudo, Hana Algül, Mehrdad Alirezaei, Iraide Alloza, Alexandru Almasan, Maylin Almonte-Beceril, Emad S. Alnemri, Covadonga Alonso, Nihal Altan-Bonnet, Dario C. Altieri, Silvia Alvarez, Lydia Alvarez-Erviti, Sandro Alves, Giuseppina Amadoro, Atsuo Amano, Consuelo Amantini, Santiago Ambrosio, Ivano Amelio, Amal O. Amer, Mohamed Amessou, Angelika Amon, Zhenyi An, Frank A. Anania, Stig U. Andersen, Usha P. Andley, Catherine K. Andreadi, Nathalie Andrieu-Abadie, Alberto Anel, David K. Ann, Shailendra Anoopkumar-Dukie, Manuela Antonioli, Hiroshi Aoki, Nadezda Apostolova, Saveria Aquila, Katia Aquilano, Koichi Araki, Eli Arama, Agustin Aranda, Jun Araya, Alexandre Arcaro, Esperanza Arias, Hirokazu Arimoto, Aileen R. Ariosa, Jane L. Armstrong, Thierry Arnould, Ivica Arsov, Katsuhiko Asanuma, Valerie Askanas, Eric Asselin, Ryuichiro Atarashi, Sally S. Atherton, Julie D. Atkin, Laura D. Attardi, Patrick Auberger, Georg Auburger, Laure Aurelian, Riccardo Autelli, Laura Avagliano, Maria Laura Avantaggiati, Limor Avrahami, Neelam Azad, Suresh Awale, Tiziana Bachetti, Jonathan M. Backer, Dong Hun Bae, Jae Sung Bae

Research output: Journal article publication › Review article › Academic research › peer-review

3606 Citations (Scopus)

Original language	English
Pages (from-to)	1-222
Number of pages	222
Journal	Autophagy
Volume	12
Issue number	1
DOIs	https://doi.org/10.1080/15548627.2015.1100356
Publication status	Published - 21 Jan 2016
Externally published	Yes

Keywords

Autolysosome
Autophagosome
Chaperonemediated autophagy
Flux
LC3
Lysosome
Macroautophagy
Phagophore
Stress
Vacuole

ASJC Scopus subject areas

Molecular Biology
Cell Biology

More information

10.1080/15548627.2015.1100356

Cite this

Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., Adhihetty, P. J., Adler, S. G., Agam, G., Agarwal, R., Aghi, M. K., Agnello, M., Agostinis, P., Aguilar, P. V., Aguirre-Ghiso, J., ... Bae, J. S. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1), 1-222. https://doi.org/10.1080/15548627.2015.1100356

Klionsky, Daniel J. ; Abdelmohsen, Kotb ; Abe, Akihisa ; Abedin, Md Joynal ; Abeliovich, Hagai ; Arozena, Abraham Acevedo ; Adachi, Hiroaki ; Adams, Christopher M. ; Adams, Peter D. ; Adeli, Khosrow ; Adhihetty, Peter J. ; Adler, Sharon G. ; Agam, Galila ; Agarwal, Rajesh ; Aghi, Manish K. ; Agnello, Maria ; Agostinis, Patrizia ; Aguilar, Patricia V. ; Aguirre-Ghiso, Julio ; Airoldi, Edoardo M. ; Ait-Si-Ali, Slimane ; Akematsu, Takahiko ; Akporiaye, Emmanuel T. ; Al-Rubeai, Mohamed ; Albaiceta, Guillermo M. ; Albanese, Chris ; Albani, Diego ; Albert, Matthew L. ; Aldudo, Jesus ; Algül, Hana ; Alirezaei, Mehrdad ; Alloza, Iraide ; Almasan, Alexandru ; Almonte-Beceril, Maylin ; Alnemri, Emad S. ; Alonso, Covadonga ; Altan-Bonnet, Nihal ; Altieri, Dario C. ; Alvarez, Silvia ; Alvarez-Erviti, Lydia ; Alves, Sandro ; Amadoro, Giuseppina ; Amano, Atsuo ; Amantini, Consuelo ; Ambrosio, Santiago ; Amelio, Ivano ; Amer, Amal O. ; Amessou, Mohamed ; Amon, Angelika ; An, Zhenyi ; Anania, Frank A. ; Andersen, Stig U. ; Andley, Usha P. ; Andreadi, Catherine K. ; Andrieu-Abadie, Nathalie ; Anel, Alberto ; Ann, David K. ; Anoopkumar-Dukie, Shailendra ; Antonioli, Manuela ; Aoki, Hiroshi ; Apostolova, Nadezda ; Aquila, Saveria ; Aquilano, Katia ; Araki, Koichi ; Arama, Eli ; Aranda, Agustin ; Araya, Jun ; Arcaro, Alexandre ; Arias, Esperanza ; Arimoto, Hirokazu ; Ariosa, Aileen R. ; Armstrong, Jane L. ; Arnould, Thierry ; Arsov, Ivica ; Asanuma, Katsuhiko ; Askanas, Valerie ; Asselin, Eric ; Atarashi, Ryuichiro ; Atherton, Sally S. ; Atkin, Julie D. ; Attardi, Laura D. ; Auberger, Patrick ; Auburger, Georg ; Aurelian, Laure ; Autelli, Riccardo ; Avagliano, Laura ; Avantaggiati, Maria Laura ; Avrahami, Limor ; Azad, Neelam ; Awale, Suresh ; Bachetti, Tiziana ; Backer, Jonathan M. ; Bae, Dong Hun ; Bae, Jae Sung. / Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). In: Autophagy. 2016 ; Vol. 12, No. 1. pp. 1-222.

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title = "Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)",

keywords = "Autolysosome, Autophagosome, Chaperonemediated autophagy, Flux, LC3, Lysosome, Macroautophagy, Phagophore, Stress, Vacuole",

author = "Klionsky, {Daniel J.} and Kotb Abdelmohsen and Akihisa Abe and Abedin, {Md Joynal} and Hagai Abeliovich and Arozena, {Abraham Acevedo} and Hiroaki Adachi and Adams, {Christopher M.} and Adams, {Peter D.} and Khosrow Adeli and Adhihetty, {Peter J.} and Adler, {Sharon G.} and Galila Agam and Rajesh Agarwal and Aghi, {Manish K.} and Maria Agnello and Patrizia Agostinis and Aguilar, {Patricia V.} and Julio Aguirre-Ghiso and Airoldi, {Edoardo M.} and Slimane Ait-Si-Ali and Takahiko Akematsu and Akporiaye, {Emmanuel T.} and Mohamed Al-Rubeai and Albaiceta, {Guillermo M.} and Chris Albanese and Diego Albani and Albert, {Matthew L.} and Jesus Aldudo and Hana Alg{\"u}l and Mehrdad Alirezaei and Iraide Alloza and Alexandru Almasan and Maylin Almonte-Beceril and Alnemri, {Emad S.} and Covadonga Alonso and Nihal Altan-Bonnet and Altieri, {Dario C.} and Silvia Alvarez and Lydia Alvarez-Erviti and Sandro Alves and Giuseppina Amadoro and Atsuo Amano and Consuelo Amantini and Santiago Ambrosio and Ivano Amelio and Amer, {Amal O.} and Mohamed Amessou and Angelika Amon and Zhenyi An and Anania, {Frank A.} and Andersen, {Stig U.} and Andley, {Usha P.} and Andreadi, {Catherine K.} and Nathalie Andrieu-Abadie and Alberto Anel and Ann, {David K.} and Shailendra Anoopkumar-Dukie and Manuela Antonioli and Hiroshi Aoki and Nadezda Apostolova and Saveria Aquila and Katia Aquilano and Koichi Araki and Eli Arama and Agustin Aranda and Jun Araya and Alexandre Arcaro and Esperanza Arias and Hirokazu Arimoto and Ariosa, {Aileen R.} and Armstrong, {Jane L.} and Thierry Arnould and Ivica Arsov and Katsuhiko Asanuma and Valerie Askanas and Eric Asselin and Ryuichiro Atarashi and Atherton, {Sally S.} and Atkin, {Julie D.} and Attardi, {Laura D.} and Patrick Auberger and Georg Auburger and Laure Aurelian and Riccardo Autelli and Laura Avagliano and Avantaggiati, {Maria Laura} and Limor Avrahami and Neelam Azad and Suresh Awale and Tiziana Bachetti and Backer, {Jonathan M.} and Bae, {Dong Hun} and Bae, {Jae Sung}",

year = "2016",

month = jan,

day = "21",

doi = "10.1080/15548627.2015.1100356",

language = "English",

volume = "12",

pages = "1--222",

journal = "Autophagy",

issn = "1554-8627",

publisher = "Landes Bioscience",

number = "1",

}

Klionsky, DJ, Abdelmohsen, K, Abe, A, Abedin, MJ, Abeliovich, H, Arozena, AA, Adachi, H, Adams, CM, Adams, PD, Adeli, K, Adhihetty, PJ, Adler, SG, Agam, G, Agarwal, R, Aghi, MK, Agnello, M, Agostinis, P, Aguilar, PV, Aguirre-Ghiso, J, Airoldi, EM, Ait-Si-Ali, S, Akematsu, T, Akporiaye, ET, Al-Rubeai, M, Albaiceta, GM, Albanese, C, Albani, D, Albert, ML, Aldudo, J, Algül, H, Alirezaei, M, Alloza, I, Almasan, A, Almonte-Beceril, M, Alnemri, ES, Alonso, C, Altan-Bonnet, N, Altieri, DC, Alvarez, S, Alvarez-Erviti, L, Alves, S, Amadoro, G, Amano, A, Amantini, C, Ambrosio, S, Amelio, I, Amer, AO, Amessou, M, Amon, A, An, Z, Anania, FA, Andersen, SU, Andley, UP, Andreadi, CK, Andrieu-Abadie, N, Anel, A, Ann, DK, Anoopkumar-Dukie, S, Antonioli, M, Aoki, H, Apostolova, N, Aquila, S, Aquilano, K, Araki, K, Arama, E, Aranda, A, Araya, J, Arcaro, A, Arias, E, Arimoto, H, Ariosa, AR, Armstrong, JL, Arnould, T, Arsov, I, Asanuma, K, Askanas, V, Asselin, E, Atarashi, R, Atherton, SS, Atkin, JD, Attardi, LD, Auberger, P, Auburger, G, Aurelian, L, Autelli, R, Avagliano, L, Avantaggiati, ML, Avrahami, L, Azad, N, Awale, S, Bachetti, T, Backer, JM, Bae, DH & Bae, JS 2016, 'Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)', Autophagy, vol. 12, no. 1, pp. 1-222. https://doi.org/10.1080/15548627.2015.1100356

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). / Klionsky, Daniel J.; Abdelmohsen, Kotb; Abe, Akihisa; Abedin, Md Joynal; Abeliovich, Hagai; Arozena, Abraham Acevedo; Adachi, Hiroaki; Adams, Christopher M.; Adams, Peter D.; Adeli, Khosrow; Adhihetty, Peter J.; Adler, Sharon G.; Agam, Galila; Agarwal, Rajesh; Aghi, Manish K.; Agnello, Maria; Agostinis, Patrizia; Aguilar, Patricia V.; Aguirre-Ghiso, Julio; Airoldi, Edoardo M.; Ait-Si-Ali, Slimane; Akematsu, Takahiko; Akporiaye, Emmanuel T.; Al-Rubeai, Mohamed; Albaiceta, Guillermo M.; Albanese, Chris; Albani, Diego; Albert, Matthew L.; Aldudo, Jesus; Algül, Hana; Alirezaei, Mehrdad; Alloza, Iraide; Almasan, Alexandru; Almonte-Beceril, Maylin; Alnemri, Emad S.; Alonso, Covadonga; Altan-Bonnet, Nihal; Altieri, Dario C.; Alvarez, Silvia; Alvarez-Erviti, Lydia; Alves, Sandro; Amadoro, Giuseppina; Amano, Atsuo; Amantini, Consuelo; Ambrosio, Santiago; Amelio, Ivano; Amer, Amal O.; Amessou, Mohamed; Amon, Angelika; An, Zhenyi; Anania, Frank A.; Andersen, Stig U.; Andley, Usha P.; Andreadi, Catherine K.; Andrieu-Abadie, Nathalie; Anel, Alberto; Ann, David K.; Anoopkumar-Dukie, Shailendra; Antonioli, Manuela; Aoki, Hiroshi; Apostolova, Nadezda; Aquila, Saveria; Aquilano, Katia; Araki, Koichi; Arama, Eli; Aranda, Agustin; Araya, Jun; Arcaro, Alexandre; Arias, Esperanza; Arimoto, Hirokazu; Ariosa, Aileen R.; Armstrong, Jane L.; Arnould, Thierry; Arsov, Ivica; Asanuma, Katsuhiko; Askanas, Valerie; Asselin, Eric; Atarashi, Ryuichiro; Atherton, Sally S.; Atkin, Julie D.; Attardi, Laura D.; Auberger, Patrick; Auburger, Georg; Aurelian, Laure; Autelli, Riccardo; Avagliano, Laura; Avantaggiati, Maria Laura; Avrahami, Limor; Azad, Neelam; Awale, Suresh; Bachetti, Tiziana; Backer, Jonathan M.; Bae, Dong Hun; Bae, Jae Sung.

In: Autophagy, Vol. 12, No. 1, 21.01.2016, p. 1-222.

Research output: Journal article publication › Review article › Academic research › peer-review

TY - JOUR

T1 - Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

AU - Klionsky, Daniel J.

AU - Abdelmohsen, Kotb

AU - Abe, Akihisa

AU - Abedin, Md Joynal

AU - Abeliovich, Hagai

AU - Arozena, Abraham Acevedo

AU - Adachi, Hiroaki

AU - Adams, Christopher M.

AU - Adams, Peter D.

AU - Adeli, Khosrow

AU - Adhihetty, Peter J.

AU - Adler, Sharon G.

AU - Agam, Galila

AU - Agarwal, Rajesh

AU - Aghi, Manish K.

AU - Agnello, Maria

AU - Agostinis, Patrizia

AU - Aguilar, Patricia V.

AU - Aguirre-Ghiso, Julio

AU - Airoldi, Edoardo M.

AU - Ait-Si-Ali, Slimane

AU - Akematsu, Takahiko

AU - Akporiaye, Emmanuel T.

AU - Al-Rubeai, Mohamed

AU - Albaiceta, Guillermo M.

AU - Albanese, Chris

AU - Albani, Diego

AU - Albert, Matthew L.

AU - Aldudo, Jesus

AU - Algül, Hana

AU - Alirezaei, Mehrdad

AU - Alloza, Iraide

AU - Almasan, Alexandru

AU - Almonte-Beceril, Maylin

AU - Alnemri, Emad S.

AU - Alonso, Covadonga

AU - Altan-Bonnet, Nihal

AU - Altieri, Dario C.

AU - Alvarez, Silvia

AU - Alvarez-Erviti, Lydia

AU - Alves, Sandro

AU - Amadoro, Giuseppina

AU - Amano, Atsuo

AU - Amantini, Consuelo

AU - Ambrosio, Santiago

AU - Amelio, Ivano

AU - Amer, Amal O.

AU - Amessou, Mohamed

AU - Amon, Angelika

AU - An, Zhenyi

AU - Anania, Frank A.

AU - Andersen, Stig U.

AU - Andley, Usha P.

AU - Andreadi, Catherine K.

AU - Andrieu-Abadie, Nathalie

AU - Anel, Alberto

AU - Ann, David K.

AU - Anoopkumar-Dukie, Shailendra

AU - Antonioli, Manuela

AU - Aoki, Hiroshi

AU - Apostolova, Nadezda

AU - Aquila, Saveria

AU - Aquilano, Katia

AU - Araki, Koichi

AU - Arama, Eli

AU - Aranda, Agustin

AU - Araya, Jun

AU - Arcaro, Alexandre

AU - Arias, Esperanza

AU - Arimoto, Hirokazu

AU - Ariosa, Aileen R.

AU - Armstrong, Jane L.

AU - Arnould, Thierry

AU - Arsov, Ivica

AU - Asanuma, Katsuhiko

AU - Askanas, Valerie

AU - Asselin, Eric

AU - Atarashi, Ryuichiro

AU - Atherton, Sally S.

AU - Atkin, Julie D.

AU - Attardi, Laura D.

AU - Auberger, Patrick

AU - Auburger, Georg

AU - Aurelian, Laure

AU - Autelli, Riccardo

AU - Avagliano, Laura

AU - Avantaggiati, Maria Laura

AU - Avrahami, Limor

AU - Azad, Neelam

AU - Awale, Suresh

AU - Bachetti, Tiziana

AU - Backer, Jonathan M.

AU - Bae, Dong Hun

AU - Bae, Jae Sung

PY - 2016/1/21

Y1 - 2016/1/21

KW - Autolysosome

KW - Autophagosome

KW - Chaperonemediated autophagy

KW - Flux

KW - LC3

KW - Lysosome

KW - Macroautophagy

KW - Phagophore

KW - Stress

KW - Vacuole

UR - http://www.scopus.com/inward/record.url?scp=85013763791&partnerID=8YFLogxK

U2 - 10.1080/15548627.2015.1100356

DO - 10.1080/15548627.2015.1100356

M3 - Review article

C2 - 26799652

VL - 12

SP - 1

EP - 222

JO - Autophagy

JF - Autophagy

SN - 1554-8627

IS - 1

ER -

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Keywords

ASJC Scopus subject areas

More information

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Cite this