Growth-inhibition effects of Cordyceps sinensis fungus extracts on HL-60 cells and inducement of G2/M arrest

Qiao Xia Zhang, Jianyong Wu, Zong Ding Hu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

The anti-tumor activities of a Cordyceps sinensis fungus extracts and the mechanism were studied in vitro. The cultivated mycelium were sequentially extracted by petroleum ether (PE), ethyl acetate (EtOAc), ethanol (EtOH) and water. The results of MTT assay show that EtOAc extract inhibits significantly the proliferation of human premyelocytic leukemia cell HL-60 with an IC50 ≤ 25 μg/mL. The mechanism of EtOAc extract inhibiting the proliferation of HL-60 was studied by flow cytometer and Western blot. The results show that HL-60 cells are arrested at the G2/M phase after treatment with 25 μg/mL of EtOAc extract for 12-24 h, and the content of p34cdc2 decreases significantly.
Original languageEnglish
Pages (from-to)581-584
Number of pages4
JournalTianjin Daxue Xuebao (Ziran Kexue yu Gongcheng Jishu Ban)/Journal of Tianjin University Science and Technology
Volume39
Issue number5
Publication statusPublished - 1 May 2006

Keywords

  • Cordyceps sinensis fungus
  • G /M arrest 2
  • Growth-inhibiting effect
  • p34 cdc2
  • Tumor cells

ASJC Scopus subject areas

  • General

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