In this work, we report a simple, rapid and sensitive approach for the electrochemical gold nanoparticle-based DNA detection with an electrocatalytic silver deposition process. The catalytic and preferential silver electrodeposition on gold nanoparticle surfaces using an indium tin oxide (ITO) electrode at certain potentials, without any chemical pretreatments of the electrode, is demonstrated. More importantly, the application of this methodology for hybridization transduction is explored. The ITO electrode surface is first coated with an electroconductive polymer, poly(2-aminobenzoic acid), to enable the chemical attachment of avidin molecules for the subsequent probe immobilization. The hybridization of the target with the probe in turn permits the binding of the gold nanoparticle labels to the transducer surface via biotin-streptavidin interaction. The amount of bound gold labels, which is proportional to the amount of the target, is determined by the electrocatalytic silver deposition process. A significant improvement of the signal-to-background ratio is achieved with this scheme compared to the conventional chemical hydroquinone-based silver deposition process.
- Catalytic silver electrodeposition
- DNA hybridization transduction
- Gold nanoparticle
ASJC Scopus subject areas
- Analytical Chemistry