Gold Nanoparticle-Based Loop-Mediated Isothermal Amplification for Rapid, Low-Cost, and Simple Detection of Salmonella in Coffee and Cream

Shiyao Wang; Eric Wai Him Lee; Jack Yiu Tat Yuen; Jackson Chit Shing Woo; Stryer Wong; Benjamin Wai Kit Chan; Shea Ping Yip; Ming Hung Thomas Lee

doi:10.1007/978-1-0716-4526-0

Gold Nanoparticle-Based Loop-Mediated Isothermal Amplification for Rapid, Low-Cost, and Simple Detection of Salmonella in Coffee and Cream

Shiyao Wang
, Eric Wai Him Lee
, Jack Yiu Tat Yuen
, Jackson Chit Shing Woo
, Stryer Wong
, Benjamin Wai Kit Chan
, Shea Ping Yip
, Ming Hung Thomas Lee

Department of Health Technology and Informatics

Research output: Chapter in book / Conference proceeding › Chapter in an edited book (as author) › Academic research

Abstract

The critical public health risk caused by Salmonella necessitates the need for efficient detection methods. Conventional techniques based on plate counting and real-time polymerase chain reaction are time-consuming, costly, and labor-intensive, thus mainly performed in centralized laboratories. To meet the emerging need for decentralized/on-site testing, here we describe a rapid, low-cost, and simple method for Salmonella detection in coffee and cream by using gold nanoparticle-based loop-mediated isothermal amplification.

Original language	English
Title of host publication	Foodborne Pathogens
Subtitle of host publication	Loop-mediated Isothermal Amplification Protocols
Publisher	Springer
Chapter	19
Pages	233-242
ISBN (Electronic)	978-1-0716-4526-0
ISBN (Print)	978-1-0716-4525-3
DOIs	https://doi.org/10.1007/978-1-0716-4526-0
Publication status	Published - 14 Jun 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Salmonella detection
loop-mediated isothermal amplification
gold nanoparticles
coffee
cream

More information

10.1007/978-1-0716-4526-0

http://hdl.handle.net/10397/113997

Cite this

Wang, S., Lee, E. W. H., Yuen, J. Y. T., Woo, J. C. S., Wong, S., Chan, B. W. K., Yip, S. P., & Lee, M. H. T. (2025). Gold Nanoparticle-Based Loop-Mediated Isothermal Amplification for Rapid, Low-Cost, and Simple Detection of Salmonella in Coffee and Cream. In Foodborne Pathogens: Loop-mediated Isothermal Amplification Protocols (pp. 233-242). Springer. https://doi.org/10.1007/978-1-0716-4526-0

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abstract = "The critical public health risk caused by Salmonella necessitates the need for efficient detection methods. Conventional techniques based on plate counting and real-time polymerase chain reaction are time-consuming, costly, and labor-intensive, thus mainly performed in centralized laboratories. To meet the emerging need for decentralized/on-site testing, here we describe a rapid, low-cost, and simple method for Salmonella detection in coffee and cream by using gold nanoparticle-based loop-mediated isothermal amplification.",

keywords = "Salmonella detection, loop-mediated isothermal amplification, gold nanoparticles, coffee, cream",

author = "Shiyao Wang and Lee, \{Eric Wai Him\} and Yuen, \{Jack Yiu Tat\} and Woo, \{Jackson Chit Shing\} and Stryer Wong and Chan, \{Benjamin Wai Kit\} and Yip, \{Shea Ping\} and Lee, \{Ming Hung Thomas\}",

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day = "14",

doi = "10.1007/978-1-0716-4526-0",

language = "English",

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Gold Nanoparticle-Based Loop-Mediated Isothermal Amplification for Rapid, Low-Cost, and Simple Detection of Salmonella in Coffee and Cream. / Wang, Shiyao; Lee, Eric Wai Him; Yuen, Jack Yiu Tat et al.
Foodborne Pathogens: Loop-mediated Isothermal Amplification Protocols. Springer, 2025. p. 233-242.

Research output: Chapter in book / Conference proceeding › Chapter in an edited book (as author) › Academic research

TY - CHAP

T1 - Gold Nanoparticle-Based Loop-Mediated Isothermal Amplification for Rapid, Low-Cost, and Simple Detection of Salmonella in Coffee and Cream

AU - Wang, Shiyao

AU - Lee, Eric Wai Him

AU - Yuen, Jack Yiu Tat

AU - Woo, Jackson Chit Shing

AU - Wong, Stryer

AU - Chan, Benjamin Wai Kit

AU - Yip, Shea Ping

AU - Lee, Ming Hung Thomas

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Y1 - 2025/6/14

N2 - The critical public health risk caused by Salmonella necessitates the need for efficient detection methods. Conventional techniques based on plate counting and real-time polymerase chain reaction are time-consuming, costly, and labor-intensive, thus mainly performed in centralized laboratories. To meet the emerging need for decentralized/on-site testing, here we describe a rapid, low-cost, and simple method for Salmonella detection in coffee and cream by using gold nanoparticle-based loop-mediated isothermal amplification.

AB - The critical public health risk caused by Salmonella necessitates the need for efficient detection methods. Conventional techniques based on plate counting and real-time polymerase chain reaction are time-consuming, costly, and labor-intensive, thus mainly performed in centralized laboratories. To meet the emerging need for decentralized/on-site testing, here we describe a rapid, low-cost, and simple method for Salmonella detection in coffee and cream by using gold nanoparticle-based loop-mediated isothermal amplification.

KW - Salmonella detection

KW - loop-mediated isothermal amplification

KW - gold nanoparticles

KW - coffee

KW - cream

U2 - 10.1007/978-1-0716-4526-0

DO - 10.1007/978-1-0716-4526-0

M3 - Chapter in an edited book (as author)

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BT - Foodborne Pathogens

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ER -

Gold Nanoparticle-Based Loop-Mediated Isothermal Amplification for Rapid, Low-Cost, and Simple Detection of Salmonella in Coffee and Cream

Abstract

UN SDGs

Keywords

More information

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