Glutamate-induced retinal lipid and protein damage: The protective effects of catechin

Andrew W. Siu, Mick K. Lau, John S. Cheng, Catherine K. Chow, Wai C. Tam, K. K. Li, Daniel K.L. Lee, Shing Shun Tony To, Chi Ho To, Chi Wai Do

Research output: Journal article publicationJournal articleAcademic researchpeer-review

13 Citations (Scopus)

Abstract

Glutamate toxicity has been implicated in various retinal diseases. Green tea leaf extract catechin has protective effects against cellular toxicity. This study investigated the effects of catechin on the glutamate-treated retina. Porcine retinal homogenates were incubated with glutamate (20 nmol) at 37 °C for 60 min. Catechin was co-incubated with the glutamate-treated retina in the same condition. The malondialdehyde (MDA) levels were determined as an index of lipid peroxidation (LPO). Differential protein expressions were derived from two-dimensional gel electrophoresis. Mass spectrometry was conducted to identify the proteins. Glutamate increased the retinal MDA (p < 0.0001) and catechin reversed the effect (p < 0.0001). There were significant changes in seven proteins after the glutamate treatment (p < 0.05), namely, heterogeneous ribonucleoprotein, thioredoxin peroxidase, 5-hydroxytryptamine receptor, pyruvate dehydrogenase, ARHA protein, peroxiredoxin 6 and proteasome. Catechin significantly reversed the changes in thioredoxin peroxidase, 5-hydroxytryptamine receptor, peroxiredoxin 6 and pyruvate dehydrogenase (p < 0.05). Our study shows that (a) retinal glutamate toxicity is mediated by LPO and protein modification, and (b) catechin ameliorates the toxicity.
Original languageEnglish
Pages (from-to)193-197
Number of pages5
JournalNeuroscience Letters
Volume432
Issue number3
DOIs
Publication statusPublished - 27 Feb 2008

Keywords

  • Catechin
  • Glutamate
  • Lipid
  • Oxidation
  • Protein
  • Retina

ASJC Scopus subject areas

  • Neuroscience(all)

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