Gleditsia sinensis fruit extract-induced apoptosis involves changes of reactive oxygen species level, mitochondrial membrane depolarization and caspase 3 activation.

Chung Hin Chui, Fung Yi Lau, Albert Sun Chi Chan, Gregory Yin Ming Cheng, Raymond Siu Ming Wong, Ka Bik Lai, Stanton Hon Lung Kok, Terry Tin Lok Au Yeung, Ivy Tuang Ngo Teo, Mable Yin Chun Yau, Filly Cheung, Chor Hing Cheng, Cheuk On Tang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

23 Citations (Scopus)

Abstract

Recently, we have shown that the anomalous fruit extract of Gleditsia sinensis (GSE) processes apoptotic activity on numerous solid tumour and leukaemia cell lines as well as primary cultured leukaemia cells obtained from bone marrow aspirate of patients. GSE treated cancer cells exhibited apoptotic features as readily illustrated by morphological investigation, DNA fragmentation analysis and TUNEL labelling methods. Elevation of intracellular superoxide dismutase activity was observed. However, the detailed mechanism still remains undefined. Here we further demonstrated that cell cycle arrest, increment of hydrogen peroxide production, changes of intracellular acid-base equilibrium and mitochondrial membrane potential depolarization (DeltaPsi(m)) were induced from cancer cells after GSE incubation. Caspase 3 protease activity was significantly enhanced upon GSE treatment. Taken together, a defined signaling pathway for the mechanistic action of GSE on cancer cells was worked out.
Original languageEnglish
Pages (from-to)539-543
Number of pages5
JournalInternational Journal of Molecular Medicine
Volume15
Issue number3
Publication statusPublished - 1 Mar 2005

ASJC Scopus subject areas

  • Genetics

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