Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray

Dieter Trau, Ming Hung Thomas Lee, Alex I K Lao, Ralf Lenigk, I. Ming Hsing, Nancy Y. Ip, Maria C. Carles, Nikolaus J. Sucher

Research output: Journal article publicationJournal articleAcademic researchpeer-review

90 Citations (Scopus)

Abstract

A novel method for the fast identification of genetic material utilizing a micro-DNA amplification and analysis device (μ-DAAD) consisting of multiple PCR microreactors with integrated DNA microarrays was developed. The device was fabricated in Si-technology and used for the genotyping of Chinese medicinal plants on the basis of differences in the noncoding region of the 5S-rRNA gene. Successful amplification of the genetic material and the consecutive analysis of the fluorescent-labeled amplicons in the μ-DAAD by the integrated oligonucleotide probes were demonstrated. Parallel analysis was performed by loading the four PCR reactors of the μ-DAAD with different samples of 3-μL volume. Temperature sensors and heating elements of the μ-DAAD enable precise temperature control and fast cycling, allowing the rapid completion of a combined amplification and analysis (hybridization) experiment.
Original languageEnglish
Pages (from-to)3168-3173
Number of pages6
JournalAnalytical Chemistry
Volume74
Issue number13
DOIs
Publication statusPublished - 1 Jul 2002
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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