Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray

Dieter Trau; Ming Hung Thomas Lee; Alex I K Lao; Ralf Lenigk; I. Ming Hsing; Nancy Y. Ip; Maria C. Carles; Nikolaus J. Sucher

doi:10.1021/ac020053u

Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray

Dieter Trau, Ming Hung Thomas Lee, Alex I K Lao, Ralf Lenigk, I. Ming Hsing, Nancy Y. Ip, Maria C. Carles, Nikolaus J. Sucher

Research output: Journal article publication › Journal article › Academic research › peer-review

97 Citations (Scopus)

Abstract

A novel method for the fast identification of genetic material utilizing a micro-DNA amplification and analysis device (μ-DAAD) consisting of multiple PCR microreactors with integrated DNA microarrays was developed. The device was fabricated in Si-technology and used for the genotyping of Chinese medicinal plants on the basis of differences in the noncoding region of the 5S-rRNA gene. Successful amplification of the genetic material and the consecutive analysis of the fluorescent-labeled amplicons in the μ-DAAD by the integrated oligonucleotide probes were demonstrated. Parallel analysis was performed by loading the four PCR reactors of the μ-DAAD with different samples of 3-μL volume. Temperature sensors and heating elements of the μ-DAAD enable precise temperature control and fast cycling, allowing the rapid completion of a combined amplification and analysis (hybridization) experiment.

Original language	English
Pages (from-to)	3168-3173
Number of pages	6
Journal	Analytical Chemistry
Volume	74
Issue number	13
DOIs	https://doi.org/10.1021/ac020053u
Publication status	Published - 1 Jul 2002
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac020053u

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title = "Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray",

abstract = "A novel method for the fast identification of genetic material utilizing a micro-DNA amplification and analysis device (μ-DAAD) consisting of multiple PCR microreactors with integrated DNA microarrays was developed. The device was fabricated in Si-technology and used for the genotyping of Chinese medicinal plants on the basis of differences in the noncoding region of the 5S-rRNA gene. Successful amplification of the genetic material and the consecutive analysis of the fluorescent-labeled amplicons in the μ-DAAD by the integrated oligonucleotide probes were demonstrated. Parallel analysis was performed by loading the four PCR reactors of the μ-DAAD with different samples of 3-μL volume. Temperature sensors and heating elements of the μ-DAAD enable precise temperature control and fast cycling, allowing the rapid completion of a combined amplification and analysis (hybridization) experiment.",

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AU - Trau, Dieter

AU - Lee, Ming Hung Thomas

AU - Lao, Alex I K

AU - Lenigk, Ralf

AU - Hsing, I. Ming

AU - Ip, Nancy Y.

AU - Carles, Maria C.

AU - Sucher, Nikolaus J.

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AB - A novel method for the fast identification of genetic material utilizing a micro-DNA amplification and analysis device (μ-DAAD) consisting of multiple PCR microreactors with integrated DNA microarrays was developed. The device was fabricated in Si-technology and used for the genotyping of Chinese medicinal plants on the basis of differences in the noncoding region of the 5S-rRNA gene. Successful amplification of the genetic material and the consecutive analysis of the fluorescent-labeled amplicons in the μ-DAAD by the integrated oligonucleotide probes were demonstrated. Parallel analysis was performed by loading the four PCR reactors of the μ-DAAD with different samples of 3-μL volume. Temperature sensors and heating elements of the μ-DAAD enable precise temperature control and fast cycling, allowing the rapid completion of a combined amplification and analysis (hybridization) experiment.

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Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray

Abstract

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