TY - JOUR
T1 - Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay
AU - Zhang, Chao
AU - Liu, Xiaonan
AU - Yao, Yao
AU - Liu, Kewu
AU - Hui, Wenli
AU - Zhu, Juanli
AU - Dou, Yaling
AU - Hua, Kai
AU - Peng, Mingli
AU - Wang, Zuankai
AU - Vermorken, Alphonsus J.M.
AU - Cui, Yali
N1 - Funding Information:
This research was supported by the National Natural Science Foundation of China (NO. 81772289 ) and Shaanxi Provincial Nano-Biomedical Detection Innovation Team Found (No. 2017KCT-25 ).
Publisher Copyright:
© 2018 The Author(s)
PY - 2018/9/28
Y1 - 2018/9/28
N2 - Developing a sensitive, low-cost, and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisticated instruments. Here, we report a general approach that enables us to detect the genotype of multiple sample types directly without DNA purification. Moreover, the PCR results can be further quantitatively analyzed based on a magnetic lateral flow assay (MLFA)system, which avoids multiple steps needed for conventional nucleic acid biosensors. As a demonstration, we show that three genotypes of aldehyde dehydrogenase 2 (ALDH2)can be identified using a small volume of sample with an accuracy of 100% and a sensitivity of 1.0 × 102 cells/μL, which are better than those of the gold standard methods. We believe that the direct PCR-MLFA system represents a significant advance toward the development of portable, sensitive biomedical platforms.
AB - Developing a sensitive, low-cost, and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisticated instruments. Here, we report a general approach that enables us to detect the genotype of multiple sample types directly without DNA purification. Moreover, the PCR results can be further quantitatively analyzed based on a magnetic lateral flow assay (MLFA)system, which avoids multiple steps needed for conventional nucleic acid biosensors. As a demonstration, we show that three genotypes of aldehyde dehydrogenase 2 (ALDH2)can be identified using a small volume of sample with an accuracy of 100% and a sensitivity of 1.0 × 102 cells/μL, which are better than those of the gold standard methods. We believe that the direct PCR-MLFA system represents a significant advance toward the development of portable, sensitive biomedical platforms.
KW - Analytical Chemistry
KW - Biochemical Analysis
KW - Clinical Genetics
KW - Fluidics
UR - http://www.scopus.com/inward/record.url?scp=85066268971&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2018.09.005
DO - 10.1016/j.isci.2018.09.005
M3 - Journal article
AN - SCOPUS:85066268971
VL - 7
SP - 170
EP - 179
JO - iScience
JF - iScience
SN - 2589-0042
ER -