TY - JOUR
T1 - Genomic driven factors enhance biocatalyst-related cellulolysis potential in anaerobic digestion
AU - Zhuang, Huichuan
AU - Lee, Po Heng
AU - Wu, Zhuoying
AU - Jing, Houde
AU - Guan, Jianyu
AU - Tang, Xiaojing
AU - Tan, Giin Yu Amy
AU - Leu, Shao Yuan
N1 - Funding Information:
The authors thank for the financial support from the Hong Kong Research Grant Council, General Research Fund (RGC/GRF15212319); Innovation and Technology Commission (GHP/042/18GD); and the Research Institute for Sustainable Urban Development (RISUD, PolyU 1-BBW6) of the Hong Kong Polytechnic University. The authors also thank Mr. W.S. Lam in the Water and Waste Analysis Laboratory and Dr. Hang Liu in the University Research Facility in Chemical and Environmental Analysis (UCEA) for sample analysis.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/8
Y1 - 2021/8
N2 - Anaerobic digestion (AD) is a promising technology to recover bioenergy from biodegradable biomass, including cellulosic wastes. Through a few fractionation/separation techniques, cellulose has demonstrated its potential in AD, but the performance of the process is rather substrate-specific, as cellulolysis bacteria are sensitive to the enzyme-substrate interactions. Cellulosome is a self-assembled enzyme complex with many functionalized modules in the bacteria which has been gradually studied, however the genomic fingerprints of the culture-specific cellulosome in AD are relatively unclear especially under processing conditions. To clarify the key factors affecting the cellulosome induced cellulolysis, this review summarized the most recent publications of AD regarding the fates of cellulose, sources and functional genes of cellulosome, and omics methods for functional analyses. Different processes for organic treatment including applying food grinds in sewer, biomass valorization, cellulose fractionation, microaeration, and enzymatic hydrolysis enhanced fermentation, were highlighted to support the sustainable development of AD technology.
AB - Anaerobic digestion (AD) is a promising technology to recover bioenergy from biodegradable biomass, including cellulosic wastes. Through a few fractionation/separation techniques, cellulose has demonstrated its potential in AD, but the performance of the process is rather substrate-specific, as cellulolysis bacteria are sensitive to the enzyme-substrate interactions. Cellulosome is a self-assembled enzyme complex with many functionalized modules in the bacteria which has been gradually studied, however the genomic fingerprints of the culture-specific cellulosome in AD are relatively unclear especially under processing conditions. To clarify the key factors affecting the cellulosome induced cellulolysis, this review summarized the most recent publications of AD regarding the fates of cellulose, sources and functional genes of cellulosome, and omics methods for functional analyses. Different processes for organic treatment including applying food grinds in sewer, biomass valorization, cellulose fractionation, microaeration, and enzymatic hydrolysis enhanced fermentation, were highlighted to support the sustainable development of AD technology.
KW - Anaerobic digestion
KW - Biomass
KW - Cellulolysis
KW - Cellulosome
KW - Cellulosome-embedded prokaryotes
UR - http://www.scopus.com/inward/record.url?scp=85104318484&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2021.125148
DO - 10.1016/j.biortech.2021.125148
M3 - Review article
C2 - 33878497
AN - SCOPUS:85104318484
SN - 0960-8524
VL - 333
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 125148
ER -
