TY - JOUR
T1 - Gallic acid suppresses cell viability, proliferation, invasion and angiogenesis in human glioma cells
AU - Lu, Yong
AU - Jiang, Feng
AU - Jiang, Hao
AU - Wu, Kalina
AU - Zheng, Xuguang
AU - Cai, Yizhong
AU - Katakowski, Mark
AU - Chopp, Michael
AU - To, Shing Shun Tony
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Gallic acid, an organic acid, also known as 3,4,5-trihydroxybenzoic acid, is cytotoxic against certain cancer cells, without harming normal cells. The objective of this study is to evaluate whether gallic acid can inhibit glioma cell viability, proliferation, invasion and reduce glioma cell mediated angiogenesis. Treatment of U87 and U251n glioma cells with gallic acid inhibited cell viability in a dose-dependent manner. BrdU and tube formation assays indicated that gallic acid significantly decreased glioma cell proliferation and tube formation in mouse brain endothelial cells, respectively. In addition, gallic acid decreased U87 cell invasion in vitro. Western blot analysis showed that expression of ADAM17, p-Akt and p-Erk was suppressed by gallic acid in both U87 and U251n cell lines. These data suggest that suppression of ADAM17 and downregulation of PI3K/Akt and Ras/MAPK signaling pathways may contribute to gallic acid-induced decrease of invasiveness. Gallic acid may be a valuable candidate for treatment of brain tumor.
AB - Gallic acid, an organic acid, also known as 3,4,5-trihydroxybenzoic acid, is cytotoxic against certain cancer cells, without harming normal cells. The objective of this study is to evaluate whether gallic acid can inhibit glioma cell viability, proliferation, invasion and reduce glioma cell mediated angiogenesis. Treatment of U87 and U251n glioma cells with gallic acid inhibited cell viability in a dose-dependent manner. BrdU and tube formation assays indicated that gallic acid significantly decreased glioma cell proliferation and tube formation in mouse brain endothelial cells, respectively. In addition, gallic acid decreased U87 cell invasion in vitro. Western blot analysis showed that expression of ADAM17, p-Akt and p-Erk was suppressed by gallic acid in both U87 and U251n cell lines. These data suggest that suppression of ADAM17 and downregulation of PI3K/Akt and Ras/MAPK signaling pathways may contribute to gallic acid-induced decrease of invasiveness. Gallic acid may be a valuable candidate for treatment of brain tumor.
KW - Angiogenesis
KW - Gallic acid
KW - Glioma
KW - Invasion
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=77954088522&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2010.05.043
DO - 10.1016/j.ejphar.2010.05.043
M3 - Journal article
C2 - 20553913
VL - 641
SP - 102
EP - 107
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2-3
ER -
