Gadolinium-europium carbonate particles: Controlled precipitation for luminescent biolabeling

Séverine Lechevallier; Pierre Lecante; Robert Mauricot; Hervé Dexpert; Jeannette Dexpert-Ghys; Hoi Kuan Kong; Ga Lai Law; Ka Leung Wong

doi:10.1021/cm102134k

Gadolinium-europium carbonate particles: Controlled precipitation for luminescent biolabeling

Séverine Lechevallier, Pierre Lecante, Robert Mauricot, Hervé Dexpert, Jeannette Dexpert-Ghys, Hoi Kuan Kong, Ga Lai Law, Ka Leung Wong

Research output: Journal article publication › Journal article › Academic research › peer-review

72 Citations (Scopus)

Abstract

Europium-doped gadolinium carbonates particles have been prepared via urea-assisted precipitation. The reaction has been followed step by step with the investigations of the precipitate by transmission electron microscopy and wide-angle X-ray scattering, in relation with infrared absorption and thermal analyses. It has been observed that spherical particles of (Gd 0.95Eu0.05)(OH)CO3, monodispersed in size and amorphous, precipitate first and then transform to agglomerated platelike crystals of (Gd0.95Eu0.05)2(CO 3)3 2H2O as the precipitation continues. The Eu3+5D0→7FJ emission spectrum and the 5D0 lifetime in the two carbonate matrices have been measured. Selected hydroxycarbonate nanoparticles (NPs), with diameters of 164 ± 20 nm, have been then transformed to oxide NPs having the cubic crystalline structure C-(Gd0.95Eu 0.05)2O3,with the same shape and size. The photoluminescence (PL) properties of hydroxycarbonate and oxide NPs, and of their colloidal suspensions in water, have been investigated. The hydroxycarbonate and the oxide NPs exhibit same PL intensities when excitation is achieved in one of the Eu3+(4f6) levels. Tests of in vitro fluorescence imaging have been performed. The luminescent NPs have been observed after their internalization by human cervical carcinoma (HeLa) cells. It is concluded that the controlled (urea-assisted) precipitation is appropriate to synthesize Gd(OH)CO3:Eu3+ and Gd2O 3:Eu3+ nanoparticles having adequate characteristics for biolabeling.

Original language	English
Pages (from-to)	6153-6161
Number of pages	9
Journal	Chemistry of Materials
Volume	22
Issue number	22
DOIs	https://doi.org/10.1021/cm102134k
Publication status	Published - 23 Nov 2010
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)
Materials Chemistry

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10.1021/cm102134k

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title = "Gadolinium-europium carbonate particles: Controlled precipitation for luminescent biolabeling",

abstract = "Europium-doped gadolinium carbonates particles have been prepared via urea-assisted precipitation. The reaction has been followed step by step with the investigations of the precipitate by transmission electron microscopy and wide-angle X-ray scattering, in relation with infrared absorption and thermal analyses. It has been observed that spherical particles of (Gd 0.95Eu0.05)(OH)CO3, monodispersed in size and amorphous, precipitate first and then transform to agglomerated platelike crystals of (Gd0.95Eu0.05)2(CO 3)3 2H2O as the precipitation continues. The Eu3+5D0→7FJ emission spectrum and the 5D0 lifetime in the two carbonate matrices have been measured. Selected hydroxycarbonate nanoparticles (NPs), with diameters of 164 ± 20 nm, have been then transformed to oxide NPs having the cubic crystalline structure C-(Gd0.95Eu 0.05)2O3,with the same shape and size. The photoluminescence (PL) properties of hydroxycarbonate and oxide NPs, and of their colloidal suspensions in water, have been investigated. The hydroxycarbonate and the oxide NPs exhibit same PL intensities when excitation is achieved in one of the Eu3+(4f6) levels. Tests of in vitro fluorescence imaging have been performed. The luminescent NPs have been observed after their internalization by human cervical carcinoma (HeLa) cells. It is concluded that the controlled (urea-assisted) precipitation is appropriate to synthesize Gd(OH)CO3:Eu3+ and Gd2O 3:Eu3+ nanoparticles having adequate characteristics for biolabeling.",

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AU - Lechevallier, Séverine

AU - Lecante, Pierre

AU - Mauricot, Robert

AU - Dexpert, Hervé

AU - Dexpert-Ghys, Jeannette

AU - Kong, Hoi Kuan

AU - Law, Ga Lai

AU - Wong, Ka Leung

PY - 2010/11/23

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N2 - Europium-doped gadolinium carbonates particles have been prepared via urea-assisted precipitation. The reaction has been followed step by step with the investigations of the precipitate by transmission electron microscopy and wide-angle X-ray scattering, in relation with infrared absorption and thermal analyses. It has been observed that spherical particles of (Gd 0.95Eu0.05)(OH)CO3, monodispersed in size and amorphous, precipitate first and then transform to agglomerated platelike crystals of (Gd0.95Eu0.05)2(CO 3)3 2H2O as the precipitation continues. The Eu3+5D0→7FJ emission spectrum and the 5D0 lifetime in the two carbonate matrices have been measured. Selected hydroxycarbonate nanoparticles (NPs), with diameters of 164 ± 20 nm, have been then transformed to oxide NPs having the cubic crystalline structure C-(Gd0.95Eu 0.05)2O3,with the same shape and size. The photoluminescence (PL) properties of hydroxycarbonate and oxide NPs, and of their colloidal suspensions in water, have been investigated. The hydroxycarbonate and the oxide NPs exhibit same PL intensities when excitation is achieved in one of the Eu3+(4f6) levels. Tests of in vitro fluorescence imaging have been performed. The luminescent NPs have been observed after their internalization by human cervical carcinoma (HeLa) cells. It is concluded that the controlled (urea-assisted) precipitation is appropriate to synthesize Gd(OH)CO3:Eu3+ and Gd2O 3:Eu3+ nanoparticles having adequate characteristics for biolabeling.

AB - Europium-doped gadolinium carbonates particles have been prepared via urea-assisted precipitation. The reaction has been followed step by step with the investigations of the precipitate by transmission electron microscopy and wide-angle X-ray scattering, in relation with infrared absorption and thermal analyses. It has been observed that spherical particles of (Gd 0.95Eu0.05)(OH)CO3, monodispersed in size and amorphous, precipitate first and then transform to agglomerated platelike crystals of (Gd0.95Eu0.05)2(CO 3)3 2H2O as the precipitation continues. The Eu3+5D0→7FJ emission spectrum and the 5D0 lifetime in the two carbonate matrices have been measured. Selected hydroxycarbonate nanoparticles (NPs), with diameters of 164 ± 20 nm, have been then transformed to oxide NPs having the cubic crystalline structure C-(Gd0.95Eu 0.05)2O3,with the same shape and size. The photoluminescence (PL) properties of hydroxycarbonate and oxide NPs, and of their colloidal suspensions in water, have been investigated. The hydroxycarbonate and the oxide NPs exhibit same PL intensities when excitation is achieved in one of the Eu3+(4f6) levels. Tests of in vitro fluorescence imaging have been performed. The luminescent NPs have been observed after their internalization by human cervical carcinoma (HeLa) cells. It is concluded that the controlled (urea-assisted) precipitation is appropriate to synthesize Gd(OH)CO3:Eu3+ and Gd2O 3:Eu3+ nanoparticles having adequate characteristics for biolabeling.

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ER -

Gadolinium-europium carbonate particles: Controlled precipitation for luminescent biolabeling

Abstract

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