Functionalized europium nanorods for in vitro imaging

Ka Leung Wong; Ga Lai Law; Margaret B. Murphy; Peter A. Tanner; Wing Tak Wong; Paul Kwan Sing Lam; Michael Hon Wah Lam

doi:10.1021/ic8000416

Functionalized europium nanorods for in vitro imaging

Ka Leung Wong, Ga Lai Law, Margaret B. Murphy, Peter A. Tanner, Wing Tak Wong, Paul Kwan Sing Lam, Michael Hon Wah Lam

Research output: Journal article publication › Journal article › Academic research › peer-review

80 Citations (Scopus)

Abstract

Emissive europium hydroxide nanorods (ENR) (20 nm x 500 nm) functionalized by a surface coating of chromophore-containing organically modified silicate (ORMOSIL) layer, have been synthesized and characterized by high-resolution transmission electron microscopy (TEM). Low-temperature photophysical characterization of the functionalized nanorods (FENR) demonstrated a strong red 5D0 luminescence both in solid and in suspended solutions. Potentials of this nanorod material for live cell imaging have also been explored. Both the bare and functionalized nanorods are able to enter living human cells with no discernible cytotoxicity. Chromophore-to-Eu 3+ energy-transfer in the functionalized nanorods enables staining of the cytoplasm of living human cells. This is confirmed by costaining with fluorescent dextran. The red chromophore-sensitized luminescence from the internalized nanorods in live human lung carcinoma cells (A549) can be observed by confocal microscopy 2 h after loading and reaches maximal emission after 24 h.

Original language	English
Pages (from-to)	5190-5196
Number of pages	7
Journal	Inorganic Chemistry
Volume	47
Issue number	12
DOIs	https://doi.org/10.1021/ic8000416
Publication status	Published - 16 Jun 2008
Externally published	Yes

ASJC Scopus subject areas

Inorganic Chemistry

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10.1021/ic8000416

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title = "Functionalized europium nanorods for in vitro imaging",

abstract = "Emissive europium hydroxide nanorods (ENR) (20 nm x 500 nm) functionalized by a surface coating of chromophore-containing organically modified silicate (ORMOSIL) layer, have been synthesized and characterized by high-resolution transmission electron microscopy (TEM). Low-temperature photophysical characterization of the functionalized nanorods (FENR) demonstrated a strong red 5D0 luminescence both in solid and in suspended solutions. Potentials of this nanorod material for live cell imaging have also been explored. Both the bare and functionalized nanorods are able to enter living human cells with no discernible cytotoxicity. Chromophore-to-Eu 3+ energy-transfer in the functionalized nanorods enables staining of the cytoplasm of living human cells. This is confirmed by costaining with fluorescent dextran. The red chromophore-sensitized luminescence from the internalized nanorods in live human lung carcinoma cells (A549) can be observed by confocal microscopy 2 h after loading and reaches maximal emission after 24 h.",

author = "Wong, {Ka Leung} and Law, {Ga Lai} and Murphy, {Margaret B.} and Tanner, {Peter A.} and Wong, {Wing Tak} and Lam, {Paul Kwan Sing} and Lam, {Michael Hon Wah}",

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language = "English",

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T1 - Functionalized europium nanorods for in vitro imaging

AU - Wong, Ka Leung

AU - Law, Ga Lai

AU - Murphy, Margaret B.

AU - Tanner, Peter A.

AU - Wong, Wing Tak

AU - Lam, Paul Kwan Sing

AU - Lam, Michael Hon Wah

PY - 2008/6/16

Y1 - 2008/6/16

N2 - Emissive europium hydroxide nanorods (ENR) (20 nm x 500 nm) functionalized by a surface coating of chromophore-containing organically modified silicate (ORMOSIL) layer, have been synthesized and characterized by high-resolution transmission electron microscopy (TEM). Low-temperature photophysical characterization of the functionalized nanorods (FENR) demonstrated a strong red 5D0 luminescence both in solid and in suspended solutions. Potentials of this nanorod material for live cell imaging have also been explored. Both the bare and functionalized nanorods are able to enter living human cells with no discernible cytotoxicity. Chromophore-to-Eu 3+ energy-transfer in the functionalized nanorods enables staining of the cytoplasm of living human cells. This is confirmed by costaining with fluorescent dextran. The red chromophore-sensitized luminescence from the internalized nanorods in live human lung carcinoma cells (A549) can be observed by confocal microscopy 2 h after loading and reaches maximal emission after 24 h.

AB - Emissive europium hydroxide nanorods (ENR) (20 nm x 500 nm) functionalized by a surface coating of chromophore-containing organically modified silicate (ORMOSIL) layer, have been synthesized and characterized by high-resolution transmission electron microscopy (TEM). Low-temperature photophysical characterization of the functionalized nanorods (FENR) demonstrated a strong red 5D0 luminescence both in solid and in suspended solutions. Potentials of this nanorod material for live cell imaging have also been explored. Both the bare and functionalized nanorods are able to enter living human cells with no discernible cytotoxicity. Chromophore-to-Eu 3+ energy-transfer in the functionalized nanorods enables staining of the cytoplasm of living human cells. This is confirmed by costaining with fluorescent dextran. The red chromophore-sensitized luminescence from the internalized nanorods in live human lung carcinoma cells (A549) can be observed by confocal microscopy 2 h after loading and reaches maximal emission after 24 h.

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SP - 5190

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JO - Inorganic Chemistry

JF - Inorganic Chemistry

IS - 12

ER -

Functionalized europium nanorods for in vitro imaging

Abstract

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