Folic acid supplementation modifies β-adrenoceptor-mediated in vitro lipolysis of obese/diabetic (+db/+db) mice

Tsz Yan Lam, Sai Wang Seto, Alice Lai Shan Au, Christina Chui Wa Poon, Rachel Wai Sum Li, Ho Yeung Lam, Wing Sze Lau, Shun Wan Chan, Sai Ming Ngai, George Pak Heng Leung, Simon Ming Yuen Lee, Stephen Kwok Wing Tsui, Yiu Wa Kwan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

10 Citations (Scopus)

Abstract

The effects of folic acid (5.7 and 71 lg/kg, 4 weeks) consumption on the β-adrenoceptors (β-ARs)-elicited lipolysis in vitro of the abdominal adipocytes of lean/control (+m/+db) and obese/diabetic (+db/+db) mice (female) were investigated. β-AR agonists (salbutamol, a β 2-AR agonist; BRL 37344 and CGP 12177, β 3-AR agonists; adrenaline, a β-AR agonist)-mediated lipolysis, β 2-, and β 3-ARs protein expression of the adipose tissues after folic acid consumption were evaluated. Our results demonstrate that a smaller magnitude of the basal (spontaneous) and the β-AR agonists-triggered lipolysis was observed in +db/+db mice, and folic acid supplementation (71 μg/kg) resulted in an improvement of both the baseline and the β-ARs-mediated lipolysis. In controls, a lower β 2- and β 3-ARs protein expression of the adipose tissues was detected in +db/+db mice, compared to +m/+db mice. In both strains fed with folic acid (71 μg/kg), a reduction of β 2-AR protein expression was observed compared to the respective controls. In +db/+db mice, folic acid (5.7 and 71 μg/kg) consumption caused a dose-dependent increase of β 3-AR protein expression compared to controls. We demonstrate that lipolysis elicited by β-AR (β 2- and β 3-ARs) agonists was blunted in +db/+db mice. Folic acid consumption has significant modulatory effects on β-ARs protein expression and lipolysis.

Original languageEnglish
Pages (from-to)1047-1055
Number of pages9
JournalExperimental Biology and Medicine
Volume234
Issue number9
DOIs
Publication statusPublished - Sep 2009

Keywords

  • β-adrenoceptor
  • +db/+db mice
  • Adipocytes
  • Folic acid
  • Lipolysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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