Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework

Shuisheng Hu, Lin Zhu, Cheong Wing Lam, Longhua Guo, Zhenyu Lin, Bin Qiu, Kwok Yin Wong, Guonan Chen, Zhenhua Liu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

12 Citations (Scopus)

Abstract

A copper(II)-based two-dimensional metal-organic framework with nanosheet structure (CuBDC NS) that possesses peroxidase (POx) mimicking activity was prepared. In the presence of hydrogen peroxide, the system catalyses the oxidation of terephthalic acid to a blue-fluorescent product (excitation = 315 nm; emission = 425 nm). Pyrophosphate has a very strong affinity for Cu 2+ ion and blocks the POx-mimicking activity of the CuBDC NS. If, however, inorganic pyrophosphatase is present, the POx mimicking activity is gradually restored because pyrophosphate is hydrolyzed. The findings were used to design a method for the determination of the activity of inorganic pyrophosphatase by fluorometry. Fluorescence increases linearly in the 1–50 mU·mL −1  inorganic pyrophosphatase activity range. The limit of detection is 0.6 mU·mL −1 (S/N = 3). [Figure not available: see fulltext.].

Original languageEnglish
Article number190
JournalMicrochimica Acta
Volume186
Issue number3
DOIs
Publication statusPublished - 1 Mar 2019

Keywords

  • CuBDC nanosheet
  • Hydrogen peroxide
  • Inhibitors
  • Peroxidase mimicking activity
  • Two-dimensional metal-organic frameworks

ASJC Scopus subject areas

  • Analytical Chemistry

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