TY - JOUR
T1 - Fluorescent Probe HKSOX-1 for imaging and detection of endogenous superoxide in live cells and in vivo
AU - Hu, Jun Jacob
AU - Wong, Nai Kei
AU - Ye, Sen
AU - Chen, Xingmiao
AU - Lu, Ming Yang
AU - Zhao, Angela Qian
AU - Guo, Yuhan
AU - Ma, Chun Hang
AU - Leung, Anskar Yu Hung
AU - Shen, Jiangang
AU - Yang, Dan
PY - 2015/6/3
Y1 - 2015/6/3
N2 - Superoxide anion radical (O2-) is undoubtedly the most important primary reactive oxygen species (ROS) found in cells, whose formation and fate are intertwined with diverse physiological and pathological processes. Here we report a highly sensitive and selective O2- detecting strategy involving O2- cleavage of an aryl trifluoromethanesulfonate group to yield a free phenol. We have synthesized three new O2- fluorescent probes (HKSOX-1, HKSOX-1r for cellular retention, and HKSOX-1m for mitochondria-targeting) which exhibit excellent selectivity and sensitivity toward O2- over a broad range of pH, strong oxidants, and abundant reductants found in cells. In confocal imaging, flow cytometry, and 96-well microplate assay, HKSOX-1r has been robustly applied to detect O2- in multiple cellular models, such as inflammation and mitochondrial stress. Additionally, our probes can be efficiently applied to visualize O2- in intact live zebrafish embryos. These probes open up exciting opportunities for unmasking the roles of O2- in health and disease.
AB - Superoxide anion radical (O2-) is undoubtedly the most important primary reactive oxygen species (ROS) found in cells, whose formation and fate are intertwined with diverse physiological and pathological processes. Here we report a highly sensitive and selective O2- detecting strategy involving O2- cleavage of an aryl trifluoromethanesulfonate group to yield a free phenol. We have synthesized three new O2- fluorescent probes (HKSOX-1, HKSOX-1r for cellular retention, and HKSOX-1m for mitochondria-targeting) which exhibit excellent selectivity and sensitivity toward O2- over a broad range of pH, strong oxidants, and abundant reductants found in cells. In confocal imaging, flow cytometry, and 96-well microplate assay, HKSOX-1r has been robustly applied to detect O2- in multiple cellular models, such as inflammation and mitochondrial stress. Additionally, our probes can be efficiently applied to visualize O2- in intact live zebrafish embryos. These probes open up exciting opportunities for unmasking the roles of O2- in health and disease.
UR - http://www.scopus.com/inward/record.url?scp=84930634442&partnerID=8YFLogxK
U2 - 10.1021/jacs.5b01881
DO - 10.1021/jacs.5b01881
M3 - Journal article
C2 - 25988218
VL - 137
SP - 6837
EP - 6843
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 21
ER -