Facile and effective separation of polysaccharides and proteins from Cordyceps sinensis mycelia by ionic liquid aqueous two-phase system

Jing Kun Yan, Hai Le Ma, Juan Juan Pei, Zhen Bin Wang, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

56 Citations (Scopus)


An ionic liquid aqueous two-phase system (ILATPS) based on the hydrophilic ionic liquid 1-butyl-3-methylimidazolium chloride ([C4mim]Cl) and inorganic salts (K3PO4and K2HPO4) was employed to separate Cordyceps sinensis polysaccharides (CSPS) and proteins by a single-step procedure. The maximum extraction efficiencies of CSPS and proteins were 89.4% and 88.2%, respectively, as obtained with the [C4mim]Cl/K3PO4system at 25 °C and pH 13.0 under optimal conditions. The partially purified CSPS obtained from the bottom salt-rich phase comprised glucose, mannose, and galactose at a molar ratio of 10:3.5:1. The backbone of CSPS mainly comprised α-1,4-linked-d-glucose residues with a molecular weight of 2.3 × 107Da. The results of this study suggest that ILATPS is an efficient and environmentally friendly system that will highlight novel possibilities in the large-scale separation and purification of active polysaccharides from fungi or mushrooms.
Original languageEnglish
Pages (from-to)278-284
Number of pages7
JournalSeparation and Purification Technology
Publication statusPublished - 15 Oct 2014


  • Aqueous two-phase system
  • Cordyceps sinensis
  • Ionic liquid
  • Polysaccharides
  • Separation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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