Abstract
An ionic liquid aqueous two-phase system (ILATPS) based on the hydrophilic ionic liquid 1-butyl-3-methylimidazolium chloride ([C4mim]Cl) and inorganic salts (K3PO4and K2HPO4) was employed to separate Cordyceps sinensis polysaccharides (CSPS) and proteins by a single-step procedure. The maximum extraction efficiencies of CSPS and proteins were 89.4% and 88.2%, respectively, as obtained with the [C4mim]Cl/K3PO4system at 25 °C and pH 13.0 under optimal conditions. The partially purified CSPS obtained from the bottom salt-rich phase comprised glucose, mannose, and galactose at a molar ratio of 10:3.5:1. The backbone of CSPS mainly comprised α-1,4-linked-d-glucose residues with a molecular weight of 2.3 × 107Da. The results of this study suggest that ILATPS is an efficient and environmentally friendly system that will highlight novel possibilities in the large-scale separation and purification of active polysaccharides from fungi or mushrooms.
Original language | English |
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Pages (from-to) | 278-284 |
Number of pages | 7 |
Journal | Separation and Purification Technology |
Volume | 135 |
DOIs | |
Publication status | Published - 15 Oct 2014 |
Keywords
- Aqueous two-phase system
- Cordyceps sinensis
- Ionic liquid
- Polysaccharides
- Separation
ASJC Scopus subject areas
- Analytical Chemistry
- Filtration and Separation