Extracellular signal-regulated kinases are not involved in activity-dependent survival or apoptosis in cerebellar granule neurons

Bin Song, Chi Ma, Shoufang Gong, Zhongmin Yuan, Dan Li, Wei Liu, Wenming Li, Ruzhu Chen, Xiaonan Zhu, Jinsheng Zeng, Yifan Han, Mingtao Li

Research output: Journal article publicationJournal articleAcademic researchpeer-review

8 Citations (Scopus)

Abstract

Cerebellar granule neurons (CGNs) depend on potassium depolarization for survival and undergo apoptosis when deprived of depolarizing concentration of potassium. Extracellular signal-regulated kinases (ERK1/2) are thought to be activated in response to potassium depolarization and responsible for the activity-dependent survival in CGNs, but one recent study has revealed that ERK1/2 is activated by potassium deprivation and is required for apoptosis of CGNs. In this study we showed that ERK1/2 was inactivated, rather than activated, by potassium deprivation, indicating a lack of ERK1/2 involvement in potassium deprivation-induced apoptosis. Furthermore, suppression of potassium depolarization-induced activation of ERK1/2 with chemical inhibitor U0126 or PD98059 had no influence on the pro-survival effect of potassium depolarisation. Thus, ERK1/2 was not required for potassium depolarization-dependent survival of CGNs. Taken together, our findings suggest that ERK1/2 is not involved in activity-dependent survival or apoptosis of CGNs.
Original languageEnglish
Pages (from-to)214-218
Number of pages5
JournalNeuroscience Letters
Volume407
Issue number3
DOIs
Publication statusPublished - 30 Oct 2006
Externally publishedYes

Keywords

  • Apoptosis
  • Cerebellar granule neurons
  • ERK1/2
  • Survival

ASJC Scopus subject areas

  • Neuroscience(all)

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