Expression of telomerase reverse transcriptase in adult goldfish retina

Wui Man Lau, George S W Tsao, K. F. So, Henry K. Yip

Research output: Journal article publicationJournal articleAcademic researchpeer-review

7 Citations (Scopus)

Abstract

Telomerase, a specialized reverse transcriptase that maintains telomere during cell division, is commonly associated with cell proliferation. Increasing evidence suggests that telomerase may bear functions other than telomere elongation. We investigated whether telomerase is expressed in the continuously growing goldfish retina. Telomeric repeat amplification protocol (TRAP) assay reveals telomerase activity in goldfish retina. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot show that telomerase catalytic subunit (TERT) is expressed at both mRNA and protein levels. Localization of TERT by immunohistochemistry indicates prominent expression of TERT in the outer nuclear layer, the inner nuclear layer, and, in a small population of cells, in the ganglion cell layer. Coexpression of TERT with proliferative cell nuclear antigen (PCNA) immunoreactivity is found in rod progenitor cells. These results suggest the role of telomerase in vertebrate central nervous system (CNS) other than telomere maintenance, such as regulation of cell cycle progression and maintenance of retinal cell phenotypes. 2007.
Original languageEnglish
Pages (from-to)160-167
Number of pages8
JournalJournal of Molecular Neuroscience
Volume32
Issue number2
DOIs
Publication statusPublished - 1 Jun 2007
Externally publishedYes

Keywords

  • Goldfish
  • PCNA
  • Retina
  • Retinal progenitor cell
  • Teleost
  • Telomerase
  • Telomerase reverse transcriptase
  • Telomeric repeats amplification protocol
  • TERT
  • TRAP

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry
  • Genetics

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