Expression and purification of Arg196 and Lys272 mutants of mevalonate kinase from Methanococcus jannaschii

Keyphrases

• Methanocaldococcus Jannaschii 100%
• Mevalonate Kinase 100%
• Kinetic Study 66%
• Active Sites 33%
• Chelates 33%
• Catalytic Activity 33%
• Structural Change 33%
• Expression Vector 33%
• Site-directed mutagenesis 33%
• Microorganisms 33%
• CD Spectroscopy 33%
• Histidine 33%
• Natural Products 33%
• Microenvironment 33%
• Essential Residue 33%
• Expression Plasmid 33%
• Affinity Column 33%
• Codon 33%
• Isoprenoids 33%
• None of the above 33%
• Bacterial Expression 33%
• Secondary Structural Elements 33%
• Metal Affinity 33%
• Salt Bridge 33%
• Large Group 33%
• 5′-end 33%

Biochemistry, Genetics and Molecular Biology

• Copurification 100%
• Methanocaldococcus jannaschii 100%
• Mevalonate Kinase 100%
• Wild Type 66%
• Enzyme 66%
• Active Site 33%
• Enzymatic Activity 33%
• Isoprenoids Biosynthesis 33%
• Plasmid 33%
• Microorganism 33%
• Site-Directed Mutagenesis 33%
• Codon 33%
• Expression Vector 33%
• Circular Dichroism 33%
• Histidine 33%