Expression and association of TRPC1 with TRPC3 during skeletal myogenesis

Kwok Kuen Cheung, Simon S. Yeung, Shannon W. Au, Levina S. Lam, Zhong Quan Dai, Ying Hui Li, Wai Ella Yeung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

17 Citations (Scopus)


Introduction: TRPC1 and TRPC3 proteins are widely expressed in skeletal muscles in forming calcium-permeable channels. Herein we characterize the expression pattern of TRPC transcripts during skeletal myogenesis in C2C12 myo-blasts. Methods: We used polymerase chain reaction and Western blotting to detect expression levels, immunohistochemistry for subcellular localization, and co-immunoprecipitation techniques to assess interaction. Results: TRPC1 localizes to the cytoplasm and is enriched in the perinuclear region in undifferentiated myoblasts. Expression of TRPC1 increases significantly during myogenesis and resides mainly in differentiated myocytes and myotubes. TRPC3 is absent in undifferentiated myoblasts, is dramatically upregulated in differentiated culture, and is preferentially expressed in myotubes. Physical interaction of TRPC1-TRPC3 was observed, suggesting the possible existence of heteromers. Conclusions: Expression of TRPC1 and TRPC3 is tightly regulated during myogensis. Evidence of TRPC1-TRPC3 interaction was first demonstrated in a muscle cell line. The functional consequences of this interaction remain to be established.
Original languageEnglish
Pages (from-to)358-365
Number of pages8
JournalMuscle and Nerve
Issue number3
Publication statusPublished - 1 Sep 2011


  • Myogenesis
  • Skeletal muscle
  • Store-operated channel
  • TRPC1
  • TRPC3

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)


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