Exploiting the potential of commercial objectives to extend the field of view of two-photon microscopy by adaptive optics

Jing Yao, Yufeng Gao, Yixuan Yin, Puxiang Lai, Shiwei Ye, Wei Zheng

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

Two-photon microscopy (TPM) has provided critical in situ and in vivo information in biomedical studies due to its high resolution, intrinsic optical sectioning, and deep penetration. However, its relatively small field of view (FOV), which is usually determined by objectives, restricts its wide application. In this paper, we propose a segment-scanning sensorless adaptive optics method to extend the FOV and achieve high-resolution and large-FOV two-photon imaging. We demonstrated the proposed method by imaging fluorescent beads, cerebral nerve cells of mouse brain slices, and cerebral vasculature and microglia of live mice. The method extended the FOV of a commercial objective from 1.8 to 3.46 mm while maintaining a lateral resolution of 840 nm and high signal-to-noise ratio. Our technology is compatible with a standard TPM and can be used for large-scale biological exploration.

Original languageEnglish
Pages (from-to)989-992
Number of pages4
JournalOptics Letters
Volume47
Issue number4
DOIs
Publication statusPublished - 15 Feb 2022

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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