Experimental Study on Enzyme-Induced Calcium Carbonate Precipitation Based on Rate Regulation at Different Temperature Environment

Hengxing Wang, Linchang Miao, Xiaohao Sun, Linyu Wu

Research output: Journal article publicationConference articleAcademic researchpeer-review


Biomineralization is a popular reinforcement technology, for soil materials in current stages, but which is rarely studied at different temperature environment. Some potential application targets are different temperature environment. Therefore, it is essential to study the feasibility of biomineralization at different temperature environment. In this article, plant urease was selected as a cost-effective enzyme for enzyme-induced carbonate precipitation (EICP) at different temperature environment. Urease activity and urease enzyme-induced carbonate precipitation were studied as a function of temperature, and N-(n-butyl)-Thiophosphoric triamide (NBPT) addition. The effects of NBPT addition on the curing efficiency of sand columns were studied by unconfined compressive strength tests and by determining the resulting concentration of CaCO3. The results demonstrated that the urease activity could be inhibited by NBPT, but the urease activity would recovered with the temperature increasing. In addition, the initial rate of calcium carbonate production would be reduced with NBPT, which could relieve the plugged port during perfusion. Therefore, the distribution of calcium carbonate in sand columns was more uniform. And the experiment has proved that NBPT was proposed as a new way to control urease activity, which resulted in a high unconfined compressive strength (UCS) of the sand columns formed at different temperature environment.

Original languageEnglish
Pages (from-to)281-290
Number of pages10
JournalGeotechnical Special Publication
Issue numberGSP 331
Publication statusPublished - Mar 2022
Externally publishedYes
Event2022 GeoCongress: State of the Art and Practice in Geotechnical Engineering - Soil Improvement, Geosynthetics, and Innovative Geomaterials - Charlotte, United States
Duration: 20 Mar 202223 Mar 2022

ASJC Scopus subject areas

  • Civil and Structural Engineering
  • Architecture
  • Building and Construction
  • Geotechnical Engineering and Engineering Geology


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