Exonuclease III-Regulated Target Cyclic Amplification-Based Single Nucleotide Polymorphism Detection Using Ultrathin Ternary Chalcogenide Nanosheets

Yanling Hu, Chaoliang Tan, Xin Lin, Zhuangchai Lai, Xiao Zhang, Qipeng Lu, Ning Feng, Dongliang Yang, Lixing Weng

Research output: Journal article publicationJournal articleAcademic researchpeer-review

2 Citations (Scopus)

Abstract

Herein, we report that the ternary chalcogenide nanosheet exhibits different affinity toward oligonucleotides with different lengths and efficiently quenches the fluorescence of dye-labeled DNA probes. Based on these findings, as a proof-of-concept application, the ternary chalcogenide nanosheet is used as a target cyclic amplification biosensor, showing high specificity in discriminating single-base mismatch. This simple strategy is fast and sensitive for the single nucleotide polymorphism detection. Ultralow detection limit of unlabeled target (250 fM) and high discrimination ratio (5%) in the mixture of perfect match (mutant-type) and single-base mismatch (wild-type) target are achieved. This sensing method is extensively compatible for the single nucleotide polymorphism detection in clinical samples, making it a promising tool for the mutation-based clinical diagnostic and genomic research.

Original languageEnglish
Article number844
JournalFrontiers in Chemistry
Volume7
DOIs
Publication statusPublished - 6 Dec 2019
Externally publishedYes

Keywords

  • fluorescent detection
  • sensor
  • single nucleotide polymorphisms
  • ternary chalcogenide nanosheets
  • two-dimensional nanomaterials

ASJC Scopus subject areas

  • Chemistry(all)

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