Evaluation of the expression of collagen type I in porous calcium phosphate ceramics implanted in an extra-osseous site

S. X. Qu, Xia Guo, J. Weng, J. C.Y. Cheng, B. Feng, H. Y. Yeung, X. D. Zhang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

32 Citations (Scopus)


The aim of the present study is to demonstrate the newly formed tissue in calcium phosphate (Ca/P) ceramics after extra-osseous implantation by histological and immunohistochemistry (IHC) methods. Synthesis porous Ca/P ceramics without adding any growth factor and living cell were implanted in the dorsal muscle of dogs for 1 and 2 months. Undecalcified and decalcified sections were stained by hematoxylin and eosin (H&E), and IHC, respectively. The histological results showed the beginning of osteogenesis and angiogenesis after being implanted for 1 month and the obvious new bone formation after being implanted for 2 months. IHC were conducted via the avidin-biotin peroxidase complex (ABC) method and the primary antibody was collagen type I. IHC results indicated that collagen type I was expressed within osteoblast-like cells and newly formed bone-like tissue in Ca/P ceramics after 1 month, and in the mineralized matrix of newly formed bone and osteoblasts, some osteocytes and some lacunae after 2 months. No cartilage and chondrocytes were observed in the histological and IHC-stained sections. Evidence of intramembranous osteogenesis was confirmed.
Original languageEnglish
Pages (from-to)659-667
Number of pages9
Issue number4
Publication statusPublished - 1 Jan 2004


  • Calcium phosphate
  • Collagen
  • Extra-osseous implantation
  • Heterotopic osteogenesis
  • Histology
  • Immunohistochemistry

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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