In the production of recombinant proteins by the insect cell-baculovirus process, fresh medium renewal prior to virus infection has been an effective measure for maintaining high protein yields. The spent medium used in the growth stage may still be unexhausted and can be reused for cell growth. In this study, we exercised the recycle of a spent insect cell medium for cell growth and recombinant protein production. It was found that the spent medium collected from a culture close to the stationary growth phase could provide full support for insect cell growth through another batch culture after fortified with suitable nutrients (yeastolate, glucose and glutamine) and a small fraction (15-20%) of fresh medium. The specific and volumetric protein yields with cells grown in the recycled spent medium were in most cases similar to those with cells grown in fresh medium. Protein yields on per unit volume of fresh medium in the process incorporating spent medium recycle compared favourably with that achieved in a batch or fed-batch process. Spent medium recycle is hence a feasible alternative for the insect cell culture-baculovirus process.
- Insect cell
- Nutrient feeding
- Spent medium recycle
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology