Central fatigue induced by excessive rehabilitation training would limit motor activity or even damage the post-stroke motor function recovery. However, the central fatigue progress during training is unclear and ignored in post-stroke rehabilitation. In this study, we tried to investigate the changes in central fatigue with fractal dimension (FD) of electromyography (EMG) at different peripheral fatigue levels based on the intracerebral haemorrhage (ICH) model. Ten Sprague-Dawley rats with ICH and EMG electrodes implantation were randomly distributed into two groups: the forced training (FOR) group with exhausted peripheral fatigue level (n=5) and fatigue-controlled (FAT) group (n=5) with peripheral fatigue constrained in moderate level. A higher central fatigue level was found in the FOR group (P<0.0001), and the central fatigue could be alleviated by peripheral fatigue-based modulation in the FAT group. The FAT group with less central fatigue achieved significantly better motor function recovery (P<0.0001), and it might be related to the recovery in the ability of motor unit recruitments.