Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Xin Ying Wang, Zhou Sheng Lai, Chung Man Yeung, Ji De Wang, Wen Deng, Hoi Yee Li, Yu Jing Han, Hsiang Fu Kung, Bo Jiang, Marie Chia Mi Lin

Research output: Journal article publicationJournal articleAcademic researchpeer-review

8 Citations (Scopus)

Abstract

Aim: To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated. Methods: A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer. Results: Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were expressed in LST-R1. The cells presented microvilli and tight junction with large nuclei. The karyotypic analysis showed hyperdiploid features with structural chromosome aberrations. The in vivo tumorigenicity was also demonstrated in nude mice xenograft experiments. The invasion assay suggested this cell line has a higher invasive ability. cDNA microarray and Western blotting show the loss of the expression of E-cadherin in LST-R1 cells. Conclusion: We established and characterized a colorectal cancer cell line, LST-R1 and LST-R1 has an obvious malignant tendency, which maybe partially attributed to the changes of the expression of some adhesion molecules, such as E-cadherin. It is also a versatile tool for exploring the original and progressive mechanisms of laterally spreading tumor and the early colon cancer genesis.

Original languageEnglish
Pages (from-to)1204-1211
Number of pages8
JournalWorld Journal of Gastroenterology
Volume14
Issue number8
DOIs
Publication statusPublished - 28 Feb 2008
Externally publishedYes

Keywords

  • Cell line
  • E-cadherin
  • Invasion
  • LST-R1

ASJC Scopus subject areas

  • Gastroenterology

Cite this