Enzyme biosensor for studying therapeutics of Alzheimer's disease

Ralf Lenigk, Edmund Lam, Ada Lai, Hong Wang, Yifan Han, Paul Carlier, Reinhard Renneberg

Research output: Journal article publicationJournal articleAcademic researchpeer-review

37 Citations (Scopus)

Abstract

An electrochemical method for the investigation and comparison of anti-Alzheimer medications that is based on the inhibition of the acetylcholinesterase is presented. The developed amperometric biosensor determines the in-vitro inhibition of the acetylcholinesterase that is co-immobilized with choline oxidase on the working electrode surface of a three-electrode system using gel entrapment. The sensor has been applied to determine the IC50values of two known and one newly developed Alzheimer remedy. A simultaneous measurement with the photometric standard method shows the applicability of our method for fast drug screening. (C) 2000 Elsevier Science S.A.
Original languageEnglish
Pages (from-to)541-547
Number of pages7
JournalBiosensors and Bioelectronics
Volume15
Issue number9-10
DOIs
Publication statusPublished - 1 Nov 2000
Externally publishedYes

Keywords

  • Acetylcholinesterase inhibitors
  • Alzheimer's disease
  • Biosensor

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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