Enhancement of Taxol production and excretion in Taxus chinensis cell culture by fungal elicitation and medium renewal

C. Wang, Jianyong Wu, X. Mei

Research output: Journal article publicationJournal articleAcademic researchpeer-review

112 Citations (Scopus)


An endophytic fungus, Aspergillus niger, isolated from the inner bark of a Taxus chinensis tree, was used as an elicitor to stimulate the Taxol (paclitaxel) production in a Taxus chinensis cell suspension culture. Different elicitor doses and elicitation times were tested in a batch culture; and the highest volumetric Taxol yield was achieved when 40 mg of the fungal elicitor (carbohydrate equivalent) l-1was added to the culture during the late exponential-growth phase. The elicitation resulted in a more than two-fold increase in the Taxol yield and about a six-fold increase in total secretion. The Taxol yield was further improved substantially by applying medium renewal and re-elicitation to the culture. In particular, with repeated medium renewal (in a way similar to medium perfusion) and a second elicitation of the culture, the volumetric Taxol yield was increased to 67.1±7.5 mg l-1, which was about seven times the amount obtained in the non-elicited batch culture. The Taxol productivity of the perfusion-like culture with repeated fungal elicitation was 1.5 mg l-1day-1, which was about 40% higher than that of the elicitor-treated batch culture and three times the productivity of the non-elicited batch culture.
Original languageEnglish
Pages (from-to)404-410
Number of pages7
JournalApplied Microbiology and Biotechnology
Issue number4
Publication statusPublished - 1 Jan 2001

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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