Enhanced bioaccessibility of cyclolinopeptides via zein-cyclodextrin nanoparticles: Simulated gastrointestinal digestion and cellular uptake study

Peifang Chen; Yong Wang; Zizhe Cai; Xuanxuan Lu

doi:10.1016/j.foodchem.2025.142841

Enhanced bioaccessibility of cyclolinopeptides via zein-cyclodextrin nanoparticles: Simulated gastrointestinal digestion and cellular uptake study

Peifang Chen, Yong Wang, Zizhe Cai, Xuanxuan Lu

Research output: Journal article publication › Journal article › Academic research › peer-review

1 Citation (Scopus)

Abstract

Cyclolinopeptides (CLS) are hydrophobic cyclic peptides in flaxseed with multiple bioactive activities. This study developed zein (Z)-cyclodextrin (CD) binary nanoparticles (NPs) as an oral delivery system for CLS. Z-CD NP had a smaller diameter (D_h) and better encapsulation effect on CLS. Formation of CLS-loaded NPs was driven by hydrogen bonds and electrostatic interactions. Presence of CD improved the thermal, pH and storage stabilities of NPs. Besides, CD prevented premature release of CLS in the stomach and enhanced the bioaccessibility of CLS to a maximum of 86.71 % ± 2.20 %. Lipid-raft-mediated endocytosis was involved in the cell uptake of NPs, where the addition of CD significantly facilitated the uptake of NPs. Z-CD NPs also enhanced absorption and reduced secretion of CLS after digestion. Overall, this study provides a simple approach to enhance the oral delivery efficiency of CLS by modulating Z-based NPs with CD.

Original language	English
Article number	142841
Journal	Food Chemistry
Volume	471
DOIs	https://doi.org/10.1016/j.foodchem.2025.142841
Publication status	Published - 15 Apr 2025
Externally published	Yes

Keywords

Cyclodextrin
Cyclolinopeptides
Nanoparticles
Oral delivery
Zein

ASJC Scopus subject areas

Analytical Chemistry
Food Science

More information

10.1016/j.foodchem.2025.142841

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@article{df0846651bf8408ebcfcb6afa0bb2b21,

title = "Enhanced bioaccessibility of cyclolinopeptides via zein-cyclodextrin nanoparticles: Simulated gastrointestinal digestion and cellular uptake study",

abstract = "Cyclolinopeptides (CLS) are hydrophobic cyclic peptides in flaxseed with multiple bioactive activities. This study developed zein (Z)-cyclodextrin (CD) binary nanoparticles (NPs) as an oral delivery system for CLS. Z-CD NP had a smaller diameter (Dh) and better encapsulation effect on CLS. Formation of CLS-loaded NPs was driven by hydrogen bonds and electrostatic interactions. Presence of CD improved the thermal, pH and storage stabilities of NPs. Besides, CD prevented premature release of CLS in the stomach and enhanced the bioaccessibility of CLS to a maximum of 86.71 \% ± 2.20 \%. Lipid-raft-mediated endocytosis was involved in the cell uptake of NPs, where the addition of CD significantly facilitated the uptake of NPs. Z-CD NPs also enhanced absorption and reduced secretion of CLS after digestion. Overall, this study provides a simple approach to enhance the oral delivery efficiency of CLS by modulating Z-based NPs with CD.",

keywords = "Cyclodextrin, Cyclolinopeptides, Nanoparticles, Oral delivery, Zein",

author = "Peifang Chen and Yong Wang and Zizhe Cai and Xuanxuan Lu",

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year = "2025",

month = apr,

day = "15",

doi = "10.1016/j.foodchem.2025.142841",

language = "English",

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TY - JOUR

T1 - Enhanced bioaccessibility of cyclolinopeptides via zein-cyclodextrin nanoparticles

T2 - Simulated gastrointestinal digestion and cellular uptake study

AU - Chen, Peifang

AU - Wang, Yong

AU - Cai, Zizhe

AU - Lu, Xuanxuan

PY - 2025/4/15

Y1 - 2025/4/15

N2 - Cyclolinopeptides (CLS) are hydrophobic cyclic peptides in flaxseed with multiple bioactive activities. This study developed zein (Z)-cyclodextrin (CD) binary nanoparticles (NPs) as an oral delivery system for CLS. Z-CD NP had a smaller diameter (Dh) and better encapsulation effect on CLS. Formation of CLS-loaded NPs was driven by hydrogen bonds and electrostatic interactions. Presence of CD improved the thermal, pH and storage stabilities of NPs. Besides, CD prevented premature release of CLS in the stomach and enhanced the bioaccessibility of CLS to a maximum of 86.71 % ± 2.20 %. Lipid-raft-mediated endocytosis was involved in the cell uptake of NPs, where the addition of CD significantly facilitated the uptake of NPs. Z-CD NPs also enhanced absorption and reduced secretion of CLS after digestion. Overall, this study provides a simple approach to enhance the oral delivery efficiency of CLS by modulating Z-based NPs with CD.

AB - Cyclolinopeptides (CLS) are hydrophobic cyclic peptides in flaxseed with multiple bioactive activities. This study developed zein (Z)-cyclodextrin (CD) binary nanoparticles (NPs) as an oral delivery system for CLS. Z-CD NP had a smaller diameter (Dh) and better encapsulation effect on CLS. Formation of CLS-loaded NPs was driven by hydrogen bonds and electrostatic interactions. Presence of CD improved the thermal, pH and storage stabilities of NPs. Besides, CD prevented premature release of CLS in the stomach and enhanced the bioaccessibility of CLS to a maximum of 86.71 % ± 2.20 %. Lipid-raft-mediated endocytosis was involved in the cell uptake of NPs, where the addition of CD significantly facilitated the uptake of NPs. Z-CD NPs also enhanced absorption and reduced secretion of CLS after digestion. Overall, this study provides a simple approach to enhance the oral delivery efficiency of CLS by modulating Z-based NPs with CD.

KW - Cyclodextrin

KW - Cyclolinopeptides

KW - Nanoparticles

KW - Oral delivery

KW - Zein

UR - https://www.scopus.com/pages/publications/85214566022

U2 - 10.1016/j.foodchem.2025.142841

DO - 10.1016/j.foodchem.2025.142841

M3 - Journal article

C2 - 39808983

AN - SCOPUS:85214566022

SN - 0308-8146

VL - 471

JO - Food Chemistry

JF - Food Chemistry

M1 - 142841

ER -

Enhanced bioaccessibility of cyclolinopeptides via zein-cyclodextrin nanoparticles: Simulated gastrointestinal digestion and cellular uptake study

Abstract

Keywords

ASJC Scopus subject areas

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