Enhanced beauvericin production with in situ adsorption in mycelial liquid culture of Fusarium redolens Dzf2

Li Jian Xu, Yuan Shuai Liu, Li Gang Zhou, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

23 Citations (Scopus)

Abstract

Fusarium redolens Dzf2, an endophytic fungal species, is a high producer of the antibiotic compound beauvericin (BEA). However, the BEA produced by the F. redolens Dzf2 fungus was retained mainly as an intracellular product. This study was to evaluate an integrated fermentation-in situ product recovery process for enhancement of BEA production in F. redolens Dzf2 myelical culture. A macroporous polystyrene resin (X-5) was selected as the sorbent and added to the mycelial culture flasks (enclosed in a nylon bag). With 2 g resin added to 40 ml medium in each flask in the early stationary growth phase (day 5), the volumetric BEA yield (on day 7) was increased from 194 to 265 mg l-1, with 65% being adsorbed to the resin phase. With resin renewal plus glucose feeding (on day 7), the BEA production was increased even more dramatically to 400 mg l-1(on day 9), double of the yield in the batch control culture. The results show that in situ adsorption was an effective strategy for enhancing the BEA production and also facilitating its recovery in the mycelial liquid culture.
Original languageEnglish
Pages (from-to)1063-1067
Number of pages5
JournalProcess Biochemistry
Volume44
Issue number10
DOIs
Publication statusPublished - 1 Oct 2009

Keywords

  • Beauvericin
  • Fermentation process
  • In situ adsorption
  • Mycelial culture
  • Polymeric resin
  • Shake-flask

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

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