Engineering Clostridia Neurotoxins with elevated catalytic activity

Jiubiao Guo, Xuehua Pan, Yanxiang Zhao, Sheng Chen

Research output: Journal article publicationJournal articleAcademic researchpeer-review

9 Citations (Scopus)

Abstract

BoNT/B and TeNT cleave substrate VAMP2 at the same scissile bond, yet these two toxins showed different efficiency on substrate hydrolysis and had different requirements for the recognition of P2' site of VAMP2, E78. These differences may be due to their different composition of their substrate recognition pockets in the active site. Swapping of LC/T S1' pocket residue, L230, with the corresponding isoleucine in LC/B increased LC/T activity by ~25 fold, while swapping of LC/B S1' pocket residue, S201, with the corresponding proline in LC/T increased LC/B activity by ~10 fold. Optimization of both S1 and S1' pocket residues of LC/T, LC/T (K168E, L230I) elevated LC/T activity by more than 100-fold. The highly active LC/T derivative engineered in this study has the potential to be used as a more effective tool to study mechanisms of exocytosis in central neuron. The LC/B derivative with elevated activity has the potential to be developed into novel therapy to minimize the impact of immunoresistance during BoNT/B therapy.
Original languageEnglish
Pages (from-to)158-166
Number of pages9
JournalToxicon
Volume74
DOIs
Publication statusPublished - 1 Nov 2013

Keywords

  • Botulinum Neurotoxin B
  • Elevated activity
  • Engineering
  • Light chain
  • TeNT
  • VAMP2

ASJC Scopus subject areas

  • Toxicology

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