TY - JOUR
T1 - Engineered hematopoietic and immune cells derived from human pluripotent stem cells
AU - Chang, Yun
AU - Hummel, Sydney N.
AU - Jung, Juhyung
AU - Jin, Gyuhyung
AU - Deng, Qing
AU - Bao, Xiaoping
N1 - Publisher Copyright:
© 2023 ISEH – Society for Hematology and Stem Cells
PY - 2023/11
Y1 - 2023/11
N2 - For the past decade, significant advances have been achieved in human hematopoietic stem cell (HSC) transplantation for treating various blood diseases and cancers. However, challenges remain with the quality control, amount, and cost of HSCs and HSC-derived immune cells. The advent of human pluripotent stem cells (hPSCs) may transform HSC transplantation and cancer immunotherapy by providing a cost-effective and scalable cell source for fundamental studies and translational applications. In this review, we discuss the current developments in the field of stem cell engineering for hematopoietic stem and progenitor cell (HSPC) differentiation and further differentiation of HSPCs into functional immune cells. The key advances in stem cell engineering include the generation of HSPCs from hPSCs, genetic modification of hPSCs, and hPSC-derived HSPCs for improved function, further differentiation of HPSCs into functional immune cells, and applications of cell culture platforms for hematopoietic cell manufacturing. Current challenges impeding the translation of hPSC-HSPCs and immune cells as well as further directions to address these challenges are also discussed.
AB - For the past decade, significant advances have been achieved in human hematopoietic stem cell (HSC) transplantation for treating various blood diseases and cancers. However, challenges remain with the quality control, amount, and cost of HSCs and HSC-derived immune cells. The advent of human pluripotent stem cells (hPSCs) may transform HSC transplantation and cancer immunotherapy by providing a cost-effective and scalable cell source for fundamental studies and translational applications. In this review, we discuss the current developments in the field of stem cell engineering for hematopoietic stem and progenitor cell (HSPC) differentiation and further differentiation of HSPCs into functional immune cells. The key advances in stem cell engineering include the generation of HSPCs from hPSCs, genetic modification of hPSCs, and hPSC-derived HSPCs for improved function, further differentiation of HPSCs into functional immune cells, and applications of cell culture platforms for hematopoietic cell manufacturing. Current challenges impeding the translation of hPSC-HSPCs and immune cells as well as further directions to address these challenges are also discussed.
UR - http://www.scopus.com/inward/record.url?scp=85171778150&partnerID=8YFLogxK
U2 - 10.1016/j.exphem.2023.08.006
DO - 10.1016/j.exphem.2023.08.006
M3 - Review article
C2 - 37611730
AN - SCOPUS:85171778150
SN - 0301-472X
VL - 127
SP - 14
EP - 27
JO - Experimental Hematology
JF - Experimental Hematology
ER -