Energy-efficient single-stage nitrite shunt denitrification with saline sewage through concise dissolved oxygen (DO) supply: Process performance and microbial communities

Huichuan Zhuang, Zhuoying Wu, Linji Xu, Shao Yuan Leu, Po Heng Lee

Research output: Journal article publicationJournal articleAcademic researchpeer-review

1 Citation (Scopus)

Abstract

Single-stage nitrite shunt denitrification (through nitrite rather than nitrate) with low dissolved oxygen (DO) supply is a better alternative in terms of energy-efficiency, short-footprint, and low C/N-ratio requirement. This study investigates the optimal DO level with temperature effect, with saline sewage at the fixed hydraulic and solids retention times of 8 h and 8 d, respectively. Moreover, 16S rRNA gene sequencing analysis corresponding with total nitrogen (TN) and chemical oxygen demand (COD) removals in each operating condition were performed. Results showed that DO of 0.3 mg/L at 20C achieved over 60.7% and over 97.9% of TN and COD removal, respectively, suggesting that such condition achieved effective nitrite-oxidizing bacteria inhibition and efficient denitrification. An unexpected finding was that sulfur-reducing Haematobacter and nitrogen-fixing Geofilum and Shinella were highly abundant with the copredominance of ammonia-oxidizing Comamonas and Nitrosomonas, nitrite-oxidizing Limnohabitans, and denitrifying Simplicispira, Castellaniella, and Nitratireductor. Further, canonical correspondence analysis (CCA) with respect to the operating conditions associated with phenotype prediction via R-based tool Tax4Fun was performed for a preliminary diagnosis of microbial functionality. The effects of DO, temperature, nitrite, and nitrate in various extents toward each predominant microbe were discussed. Collectively, DO is likely pivotal in single-stage nitrite shunt denitrification, as well as microbial communities, for energy-efficient saline sewage treatment.

Original languageEnglish
Article number919
Pages (from-to)1-13
Number of pages13
JournalMicroorganisms
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 2020

Keywords

  • 16S rRNA sequencing
  • Dissolve oxygen
  • Nitrite shunt denitrification
  • Total nitrogen removal

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Microbiology (medical)

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