Endocrine gland-derived vascular endothelial growth factor stimulates proliferation and tube formation in human uterine microvascular endothelial cell through the mitogen-activated protein kinase but not through the Akt pathway

Yin Lau Lee, Yuk Ling Chan, Wan Ngai Chow, Ernest Hung Yu Ng, Kai Fai Lee, William S.B. Yeung, Pak Chung Ho

Research output: Journal article publicationJournal articleAcademic researchpeer-review

11 Citations (Scopus)

Abstract

Objective: To study the angiogenic functions of endocrine gland-derived vascular endothelial growth factor (EG-VEGF) on a normal myometrial uterine microvascular endothelial cell line (UtMVEC-Myo) and the signaling pathways elicited by EG-VEGF in UtMVEC-Myo. Design: Experimental laboratory study. Setting: University gynecology unit. Patient(s): Infertile women undergoing diagnostic laparoscopy for assessment of tubal patency. Intervention(s): Real-time polymerase chain reaction (PCR) analysis of mRNA of EG-VEGF and its receptors, PKR1 and PKR2, in UtMVEC-Myo and endometrial samples. The effects of EG-VEGF on the cell proliferation, tube formation, and cell signaling pathways of UtMVEC-Myo were studied. Main Outcome Measure(s): Cell proliferation, tube formation, and molecules of cell-signaling pathways in the treated UtMVEC-Myo. Result(s): UtMVEC-Myo cells had PKR1 and PKR2 but not EG-VEGF mRNA. EG-VEGF significantly stimulated cell proliferation and tube formation in UtMVEC-Myo cells. EG-VEGF activated p44/42 mitogen-activated protein kinase (MAPK) but not Akt signaling pathway. The effects of EG-VEGF on p44/42 MAPK phosphorylation and cell proliferation were nullified by the specific MAPK inhibitor, PD98059. Conclusion(s): EG-VEGF has a direct angiogenic effect on UtMVEC-Myo that expresses EG-VEGF receptors (PKR1 and PKR2) and modulates cell proliferation and sprouting of the endothelial cells. It is suggested that EG-VEGF enhanced cell proliferation through the activation of MAPK pathway but not through the Akt pathway.

Original languageEnglish
Pages (from-to)2163-2171
Number of pages9
JournalFertility and Sterility
Volume91
Issue number5 SUPPL.
Early online date20 Jun 2008
DOIs
Publication statusPublished - May 2009
Externally publishedYes

Keywords

  • angiogenesis
  • cell-signaling pathways
  • EG-VEGF
  • PKR1
  • PKR2
  • UtMVEC-Myo

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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