Emergence of tet(X4)-positive hypervirulent Klebsiella pneumoniae of food origin in China

Yan Li; Zhiqiang Wang; Huiyue Dong; Mianzhi Wang; Shangshang Qin; Sheng Chen; Ruichao Li

doi:10.1016/j.lwt.2022.114280

Emergence of tet(X4)-positive hypervirulent Klebsiella pneumoniae of food origin in China

Yan Li, Zhiqiang Wang, Huiyue Dong, Mianzhi Wang, Shangshang Qin, Sheng Chen, Ruichao Li

Research output: Journal article publication › Journal article › Academic research › peer-review

3 Citations (Scopus)

Abstract

The emergence of hypervirulent Klebsiella pneumoniae (hvKp) poses a great threat to human health, while tigecycline provides an important guarantee for the treatment of MDR-hvKp infections. Currently, prevalence of tet(X4)-positive hvKp in animal origin remains unknown. Six tet(X4)-positive K. pneumoniae isolates were isolated from pork samples in China. Notably, one of them was identified as a hvKp isolate. This isolate has a hypermucoviscous and a hypervirulent phenotype according to string test and mouse infection model. The tet(X4) gene in six K. pneumoniae isolates was located on an IncFII(pCRY) plasmid. Interestingly, the backbone structure of IncFII(pCRY) tet(X4)-positive plasmid was similar to that of bla_KPC-2-positive plasmid in hvKp. Besides, this IncFII(pCRY) plasmid can be transferred into E. coli and ST11 hvKp, and does not incur a significant fitness cost, and is able to persist stably. A novel tet(X4) core genetic environment (abh-tet(X4)-IS102-1-ISCR2) was found on the plasmid. To the best of our knowledge, this is the first report of tet(X4)-positive hvKp in animals. Our findings highlight the urgent need for further global surveillance of tet(X4)-positive hvKp in clinical and animal farming settings with the One Health approach.

Original language	English
Article number	114280
Journal	LWT
Volume	173
DOIs	https://doi.org/10.1016/j.lwt.2022.114280
Publication status	Published - 1 Jan 2023
Externally published	Yes

Keywords

Hypervirulence
Klebsiella pneumonia
Multidrug resistance
Pork
tet(X4)

ASJC Scopus subject areas

Food Science

More information

10.1016/j.lwt.2022.114280

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@article{e212a4c7d2f4468383160ab2324a27e3,

title = "Emergence of tet(X4)-positive hypervirulent Klebsiella pneumoniae of food origin in China",

abstract = "The emergence of hypervirulent Klebsiella pneumoniae (hvKp) poses a great threat to human health, while tigecycline provides an important guarantee for the treatment of MDR-hvKp infections. Currently, prevalence of tet(X4)-positive hvKp in animal origin remains unknown. Six tet(X4)-positive K. pneumoniae isolates were isolated from pork samples in China. Notably, one of them was identified as a hvKp isolate. This isolate has a hypermucoviscous and a hypervirulent phenotype according to string test and mouse infection model. The tet(X4) gene in six K. pneumoniae isolates was located on an IncFII(pCRY) plasmid. Interestingly, the backbone structure of IncFII(pCRY) tet(X4)-positive plasmid was similar to that of blaKPC-2-positive plasmid in hvKp. Besides, this IncFII(pCRY) plasmid can be transferred into E. coli and ST11 hvKp, and does not incur a significant fitness cost, and is able to persist stably. A novel tet(X4) core genetic environment (abh-tet(X4)-IS102-1-ISCR2) was found on the plasmid. To the best of our knowledge, this is the first report of tet(X4)-positive hvKp in animals. Our findings highlight the urgent need for further global surveillance of tet(X4)-positive hvKp in clinical and animal farming settings with the One Health approach.",

keywords = "Hypervirulence, Klebsiella pneumonia, Multidrug resistance, Pork, tet(X4)",

author = "Yan Li and Zhiqiang Wang and Huiyue Dong and Mianzhi Wang and Shangshang Qin and Sheng Chen and Ruichao Li",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China ( 32161133005 ), Postgraduate Research & Practice Innovation Program of Jiangsu Province ( SJCX22_1803 ) and the Priority Academic Program Development of Jiangsu Higher Education Institutions ( PAPD ). Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2023",

month = jan,

day = "1",

doi = "10.1016/j.lwt.2022.114280",

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TY - JOUR

T1 - Emergence of tet(X4)-positive hypervirulent Klebsiella pneumoniae of food origin in China

AU - Li, Yan

AU - Wang, Zhiqiang

AU - Dong, Huiyue

AU - Wang, Mianzhi

AU - Qin, Shangshang

AU - Chen, Sheng

AU - Li, Ruichao

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China ( 32161133005 ), Postgraduate Research & Practice Innovation Program of Jiangsu Province ( SJCX22_1803 ) and the Priority Academic Program Development of Jiangsu Higher Education Institutions ( PAPD ). Publisher Copyright: © 2022 The Authors

PY - 2023/1/1

Y1 - 2023/1/1

N2 - The emergence of hypervirulent Klebsiella pneumoniae (hvKp) poses a great threat to human health, while tigecycline provides an important guarantee for the treatment of MDR-hvKp infections. Currently, prevalence of tet(X4)-positive hvKp in animal origin remains unknown. Six tet(X4)-positive K. pneumoniae isolates were isolated from pork samples in China. Notably, one of them was identified as a hvKp isolate. This isolate has a hypermucoviscous and a hypervirulent phenotype according to string test and mouse infection model. The tet(X4) gene in six K. pneumoniae isolates was located on an IncFII(pCRY) plasmid. Interestingly, the backbone structure of IncFII(pCRY) tet(X4)-positive plasmid was similar to that of blaKPC-2-positive plasmid in hvKp. Besides, this IncFII(pCRY) plasmid can be transferred into E. coli and ST11 hvKp, and does not incur a significant fitness cost, and is able to persist stably. A novel tet(X4) core genetic environment (abh-tet(X4)-IS102-1-ISCR2) was found on the plasmid. To the best of our knowledge, this is the first report of tet(X4)-positive hvKp in animals. Our findings highlight the urgent need for further global surveillance of tet(X4)-positive hvKp in clinical and animal farming settings with the One Health approach.

AB - The emergence of hypervirulent Klebsiella pneumoniae (hvKp) poses a great threat to human health, while tigecycline provides an important guarantee for the treatment of MDR-hvKp infections. Currently, prevalence of tet(X4)-positive hvKp in animal origin remains unknown. Six tet(X4)-positive K. pneumoniae isolates were isolated from pork samples in China. Notably, one of them was identified as a hvKp isolate. This isolate has a hypermucoviscous and a hypervirulent phenotype according to string test and mouse infection model. The tet(X4) gene in six K. pneumoniae isolates was located on an IncFII(pCRY) plasmid. Interestingly, the backbone structure of IncFII(pCRY) tet(X4)-positive plasmid was similar to that of blaKPC-2-positive plasmid in hvKp. Besides, this IncFII(pCRY) plasmid can be transferred into E. coli and ST11 hvKp, and does not incur a significant fitness cost, and is able to persist stably. A novel tet(X4) core genetic environment (abh-tet(X4)-IS102-1-ISCR2) was found on the plasmid. To the best of our knowledge, this is the first report of tet(X4)-positive hvKp in animals. Our findings highlight the urgent need for further global surveillance of tet(X4)-positive hvKp in clinical and animal farming settings with the One Health approach.

KW - Hypervirulence

KW - Klebsiella pneumonia

KW - Multidrug resistance

KW - Pork

KW - tet(X4)

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U2 - 10.1016/j.lwt.2022.114280

DO - 10.1016/j.lwt.2022.114280

M3 - Journal article

AN - SCOPUS:85144600649

SN - 0023-6438

VL - 173

JO - LWT

JF - LWT

M1 - 114280

ER -

Emergence of tet(X4)-positive hypervirulent Klebsiella pneumoniae of food origin in China

Abstract

Keywords

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