Embryonic stem cells biosensor and its application in drug analysis and toxin detection

Qingjun Liu, Hua Cai, Lidan Xiao, Rong Li, Mo Yang, Ping Wang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

11 Citations (Scopus)

Abstract

To investigate the use of stem cells as biosensor elements, a novel cell-based light-addressable Potentiometric sensor (LAPS) was developed for monitoring cellular beating. Mouse embryonic stem cells were induced to differentiate into cardiomyocytes in vitro. Extracellular field potentials of spontaneously beating cardiomyocytes induced from stem cells were recorded by LAPS in the potential and frequency ranges of 25-45 μV and 0.5-3 Hz, respectively. Due to its capability of monitoring important physiological parameters such as potential and frequency in vitro, the sensor can be used in drug analysis and toxin detection in a long-term and noninvasive way. The pharmacological and toxicological researches make it possible to use stem cells-based biosensor for biomedical assays.
Original languageEnglish
Pages (from-to)1625-1630
Number of pages6
JournalIEEE Sensors Journal
Volume7
Issue number12
DOIs
Publication statusPublished - 1 Dec 2007

Keywords

  • Biosensor
  • Cardiomyocyte
  • Drug analysis
  • Embryonic stem cells
  • Light-addressable Potentiometrie sensor (LAPS)
  • Toxin detection

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

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