Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Salvia miltiorrhiza hairy roots

Qiong Yan, Ming Shi, Janet Ng, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

157 Citations (Scopus)


This work is to examine the rosmarinic acid (RA) accumulation and related secondary metabolism enzyme activities of Salvia miltiorrhiza Bunge (Lamiacae) hairy roots in response to a biotic elicitor, yeast extract (YE) and an abiotic elicitor, Ag+. RA accumulation as well as total phenolic content of the hairy roots was increased by both elicitors, but more significantly by YE. Preceding the elicitor-induced RA accumulation, there was a notable rise in the tyrosine aminotransferase (TAT) activity but a rapid drop in the phenylalanine ammonia-lyase (PAL) activity of roots. All elicitor effects were dependent on the elicitor dose, maximized with YE at 200 mg/L and Ag+at 15 μM. The results showed that the elicitor-induced biosynthesis of RA and phenolic compounds in the S. miltiorrhiza hairy roots was correlated with the TAT but not the PAL activity.
Original languageEnglish
Pages (from-to)853-858
Number of pages6
JournalPlant Science
Issue number4
Publication statusPublished - 1 Apr 2006


  • Elicitors
  • Hairy root culture
  • Phenylalanine ammonia-lyase
  • Rosmarinic acid
  • Salvia miltiorrhiza
  • Tyrosine aminotransferase

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

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