Elfin is expressed during early heart development

Masayo Kotaka, Yee Man Lau, Kwok Kuen Cheung, Simon M.Y. Lee, Hoi Yeung Li, Wood Yee Chan, Kwok Pui Fung, Cheuk Yu Lee, Mary M.Y. Waye, Stephen K.W. Tsui

Research output: Journal article publicationJournal articleAcademic researchpeer-review

30 Citations (Scopus)

Abstract

Elfin (previously named CLIM1) is a protein that possesses an N-terminal PDZ domain and a C-terminal LIM domain. It belongs to the family of Enigma proteins. Enigma proteins are a family of Cytoplasmic proteins that contain an N-terminal PDZ domain and a series of C-terminal LIM domains. By virtue of these two protein interacting domains, Enigma proteins are capable of protein-protein interactions. It has been proposed that Enigma proteins may act as adapters between kinases and the cytoskeleton. We have previously shown that Elfin is most abundantly expressed in the heart and it colocalizes with α-actinin 2 at the Z-disks of the myocardium. In this report, Elfin was shown to localize at the actin stress fibers of myoblasts, as revealed by green fluorescent protein (GFP) tagging. In situ hybridization and immunostaining showed that Elfin expression begins at an early stage in mouse development and is present throughout the developing heart. Taken together, our experimental results suggest that Elfin may play an important role in myofibrillogenesis and heart development.

Original languageEnglish
Pages (from-to)463-472
Number of pages10
JournalJournal of Cellular Biochemistry
Volume83
Issue number3
DOIs
Publication statusPublished - 1 Jan 2001
Externally publishedYes

Keywords

  • CLIM1
  • CLP-36
  • Embryonic development
  • Enigma protein
  • Heart muscle
  • LIM domain
  • PDZ domain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this