Electrochemical real-time polymerase chain reaction

S. S W Yeung, Ming Hung Thomas Lee, I. Ming Hsing

Research output: Journal article publicationJournal articleAcademic researchpeer-review

67 Citations (Scopus)

Abstract

In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon the advantages of the well-established fluorescence-based counterpart, such as short assay time (simultaneous target DNA amplification and detection). In addition, this electrochemical approach could employ simple and miniaturizable instrumentation compared to the bulky and expensive optics required in the fluorescence-based schemes. We have demonstrated a proof-of-concept experiment showing that the utilization of solid-phase extension of the electrode surface-immobilized capture probe with Fc-dUTP during PCR resulted in the accumulation of the redox marker on the transducer surface. This new technique can be applied to a microfabricated PCR electrochemical device for point-of-care diagnostics as well as on-site environmental monitoring and biowarfare agent detection.
Original languageEnglish
Pages (from-to)13374-13375
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number41
DOIs
Publication statusPublished - 18 Oct 2006

ASJC Scopus subject areas

  • Chemistry(all)

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