Electrochemical real-time PCR

Siu Wai Yeung; Ming Hung Thomas Lee; I. Ming Hsing

Electrochemical real-time PCR

Siu Wai Yeung, Ming Hung Thomas Lee, I. Ming Hsing

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

Abstract

In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon all advantages of the well-established fluorescence-based counterpart such as short assay time (simultaneous target DNA amplification and detection). In addition, this electrochemical approach utilizes simple and miniaturizable instrumentation compared to the bulky and expensive optics required in the fluorescence-based schemes.

Original language	English
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Japan Academic Association Inc
Pages	711-713
Number of pages	3
Publication status	Published - 1 Jan 2006
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 5 Nov 2006 → 9 Nov 2006

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	5/11/06 → 9/11/06

Keywords

Electrochemistry
Ferrocene-dUTP
Real-time PCR
Solid-phase extension

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "Electrochemical real-time PCR",

abstract = "In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon all advantages of the well-established fluorescence-based counterpart such as short assay time (simultaneous target DNA amplification and detection). In addition, this electrochemical approach utilizes simple and miniaturizable instrumentation compared to the bulky and expensive optics required in the fluorescence-based schemes.",

keywords = "Electrochemistry, Ferrocene-dUTP, Real-time PCR, Solid-phase extension",

author = "Yeung, {Siu Wai} and Lee, {Ming Hung Thomas} and Hsing, {I. Ming}",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "711--713",

booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Japan Academic Association Inc",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

}

Yeung, SW, Lee, MHT & Hsing, IM 2006, Electrochemical real-time PCR. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 711-713, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 5/11/06.

Electrochemical real-time PCR. / Yeung, Siu Wai; Lee, Ming Hung Thomas; Hsing, I. Ming.
Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 711-713.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

TY - GEN

T1 - Electrochemical real-time PCR

AU - Yeung, Siu Wai

AU - Lee, Ming Hung Thomas

AU - Hsing, I. Ming

PY - 2006/1/1

Y1 - 2006/1/1

N2 - In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon all advantages of the well-established fluorescence-based counterpart such as short assay time (simultaneous target DNA amplification and detection). In addition, this electrochemical approach utilizes simple and miniaturizable instrumentation compared to the bulky and expensive optics required in the fluorescence-based schemes.

AB - In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon all advantages of the well-established fluorescence-based counterpart such as short assay time (simultaneous target DNA amplification and detection). In addition, this electrochemical approach utilizes simple and miniaturizable instrumentation compared to the bulky and expensive optics required in the fluorescence-based schemes.

KW - Electrochemistry

KW - Ferrocene-dUTP

KW - Real-time PCR

KW - Solid-phase extension

UR - http://www.scopus.com/inward/record.url?scp=84902491368&partnerID=8YFLogxK

M3 - Conference article published in proceeding or book

SP - 711

EP - 713

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Japan Academic Association Inc

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Electrochemical real-time PCR

Abstract

Conference

Keywords

ASJC Scopus subject areas

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