Electrochemical real-time PCR

Siu Wai Yeung, Ming Hung Thomas Lee, I. Ming Hsing

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

Abstract

In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon all advantages of the well-established fluorescence-based counterpart such as short assay time (simultaneous target DNA amplification and detection). In addition, this electrochemical approach utilizes simple and miniaturizable instrumentation compared to the bulky and expensive optics required in the fluorescence-based schemes.
Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages711-713
Number of pages3
Publication statusPublished - 1 Jan 2006
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 5 Nov 20069 Nov 2006

Conference

Conference10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/TerritoryJapan
CityTokyo
Period5/11/069/11/06

Keywords

  • Electrochemistry
  • Ferrocene-dUTP
  • Real-time PCR
  • Solid-phase extension

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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