Electrical assisted patterning of cardiac myocytes with controlled macroscopic anisotropy using a microfluidic dielectrophoresis chip

Mo Yang, Xin Zhang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

38 Citations (Scopus)

Abstract

Cardiac tissue engineering has evolved as a potential therapeutic approach to assist cardiac regeneration. Controlling the preferential cell orientation of engineered heart tissues is a key issue in cardiac tissue engineering. Here, we present a novel method to construct a model-engineered cardiac tissue-like structure with anisotropic properties. Our analysis shows that the electro-torque which acts on a cylindrical or rod shape cell is zero whenever the electric field is aligned with one of its principal axes. With the interdigitated-castellated microelectrodes, the induction of dielectrophoresis and electro-orientation can accumulate cells and form a tissue-like structure with orientation along the ac electric field. Both experiments and analysis indicate that a large orientation torque and force can be achieved with appropriate frequency and low conductive medium. Finally, we report basic structural and biophysical anisotropy of electro-oriented structure through electromechanical experiments.
Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalSensors and Actuators, A: Physical
Volume135
Issue number1
DOIs
Publication statusPublished - 30 Mar 2007

Keywords

  • Controlled anisotropy
  • Dielectrophoresis
  • Electro-orientation
  • Microfluidic chip

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering

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