Electric Field-Assisted Positioning of Neurons on Pt Microelectrode Arrays

Shalini Prasad, Mo Yang, Xuan Zhang, Yingchun Ni, Vladimir Parpura, Cengiz S. Ozkan, Mihrimah Ozkan

Research output: Journal article publicationConference articleAcademic researchpeer-review

Abstract

Characterization of electrical activity of individual neurons is the fundamental step in understanding the functioning of the nervous system Single cell electrical activity at various stages of cell development is essential to accurately determine in in-vivo conditions the position of a cell based on the procured electrical activity. Understanding memory formation and development translates to changes in the electrical activity of individual neurons. Hence, there is an enormous need to develop novel ways for isolating and positioning individual neurons over single recording sites. To this end, we used a 3×3 multiple microelectrode array system to spatially arrange neurons by applying a gradient AC field. We characterized the electric field distribution inside our test platform by using two dimensional finite element modeling (FEM) and determined the location of neurons over the electrode array. Dielectrophoretic AC fields were utilized to separate the neurons from the glial cells and to position the neurons over the electrodes. The neurons were obtained from 0-2-day-old rat (Sprague-Dawley) pups. The technique of using electric fields to achieve single neuron patterning has implications in neural engineering, elucidating a new and simpler method to develop and study neuronal activity as compared to conventional microelectrode array techniques.
Original languageEnglish
Pages (from-to)3-11
Number of pages9
JournalMaterials Research Society Symposium - Proceedings
Volume773
Publication statusPublished - 1 Dec 2003
Externally publishedYes
EventBiomicroelectromechanical Systems (BioMEMS) - San Francisco, CA, United States
Duration: 22 Apr 200325 Apr 2003

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

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