Efficient saccharification of wheat straw pretreated by solid particle-assisted ball milling with waste washing liquor recycling

Xiao le Liu, Chengyu Dong, Shao Yuan Leu, Zhen Fang, Zheng diao Miao

Research output: Journal article publicationJournal articleAcademic researchpeer-review


Wheat straw was pretreated using ball milling (BM) promoted by solid particles (NaOH, NaCl, citric acid). NaOH showed the best synergistic interaction effect, due to the breakage of β-1,4-glycosidic bonds among cellulose molecules by the alkali solid particles induced by BM. NaOH-BM pretreatment decreased the straw crystallinity from 46% to 21.4% and its average particle size from 398.3 to 50.6 μm in 1 h. After 4 h milling, the reducing-end concentration of cellulose increased by 3.8 times from 12.5 to 60.2 μM, with glucose yield increased by 2.1 times from 26.6% to 82.4% for 72 h enzymatic hydrolysis at cellulase loading of 15 FPU/g dry substrate. The pretreatment washing liquor was recycled for the re-treatment of partially pretreated biomass at 121 °C for 30 min, resulting in 99.4% glucose yield by enzymatic hydrolysis. BM assisted with alkali particles was an effective approach for improving biomass enzymatic saccharification.

Original languageEnglish
Article number126721
JournalBioresource Technology
Publication statusPublished - Mar 2022


  • Ball milling
  • Enzymatic hydrolysis
  • Pretreatment
  • Solid particle
  • Wheat straw

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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