TY - JOUR
T1 - Efficient isolation of immunostimulatory polysaccharides from Lentinula edodes by autoclaving-ultrasonication extraction and fractional precipitation
AU - Li, Jun Hui
AU - Zhu, Yan Yu
AU - Gu, Fang Ting
AU - Wu, Jian Yong
N1 - Funding Information:
This work was financially supported by the Hong Kong Polytechnic University through RI-Food Project ( 1-CD59 ) and Joint Postdoc Scheme with Non-local Institutions ( 1-YY4C ). We also gratefully acknowledge the support of the University Research Facility on Chemical and Environmental Analysis (UCEA) of PolyU.
Publisher Copyright:
© 2023
PY - 2023/5/15
Y1 - 2023/5/15
N2 - A hyphenated process, autoclaving followed by ultrasonication (AU), was evaluated for efficient extraction of polysaccharides (PS) from Lentinula edodes (Shiitake) mushroom. The PS yield (w/w) was 8.44 % from hot-water extraction (HWE), 11.01 % by autoclaving extraction (AE), and 16.3 % by AUE. The AUE water extract was subject to fractional precipitation in four-steps with increasing ethanol concentration of 40 %, 50 %, 70 % and 80 % (v/v), yielding four PS fractions in descending molecular weight (MW), PS40 > PS50 > PS70 > PS80. All the four PS fractions were composed of four monosaccharide residues, mannose (Man), glucose (Glc) and galactose (Gal) but in different mole ratios. The PS40 fraction with the highest average MW (4.98 × 106) was the most abundant fraction, accounting for 64.4 % of the total PS mass and also had the highest glucose molar ratio (~80 %). PS40 also most significantly enhanced the NO, and ROS generation and phagocytic activity in RAW 264.7 cells. The results proved that AUE followed by fractional ethanol precipitation is an efficient strategy with reduced solvent expenditure for isolation of the major immunostimulatory PS from L. edodes mushroom.
AB - A hyphenated process, autoclaving followed by ultrasonication (AU), was evaluated for efficient extraction of polysaccharides (PS) from Lentinula edodes (Shiitake) mushroom. The PS yield (w/w) was 8.44 % from hot-water extraction (HWE), 11.01 % by autoclaving extraction (AE), and 16.3 % by AUE. The AUE water extract was subject to fractional precipitation in four-steps with increasing ethanol concentration of 40 %, 50 %, 70 % and 80 % (v/v), yielding four PS fractions in descending molecular weight (MW), PS40 > PS50 > PS70 > PS80. All the four PS fractions were composed of four monosaccharide residues, mannose (Man), glucose (Glc) and galactose (Gal) but in different mole ratios. The PS40 fraction with the highest average MW (4.98 × 106) was the most abundant fraction, accounting for 64.4 % of the total PS mass and also had the highest glucose molar ratio (~80 %). PS40 also most significantly enhanced the NO, and ROS generation and phagocytic activity in RAW 264.7 cells. The results proved that AUE followed by fractional ethanol precipitation is an efficient strategy with reduced solvent expenditure for isolation of the major immunostimulatory PS from L. edodes mushroom.
KW - Autoclaving-ultrasonication extraction
KW - Immunostimulatory activity
KW - Lentinula edodes
KW - Molecular weight
KW - Polysaccharide
UR - http://www.scopus.com/inward/record.url?scp=85151030246&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.124216
DO - 10.1016/j.ijbiomac.2023.124216
M3 - Journal article
C2 - 36990414
AN - SCOPUS:85151030246
SN - 0141-8130
VL - 237
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 124216
ER -