Effects of Tween 80 and pH on mycelial pellets and exopolysaccharide production in liquid culture of a medicinal fungus

Yuan Shuai Liu, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

41 Citations (Scopus)


This study investigated the eVects of surfactant additives and medium pH on mycelial morphology and exopolysaccharide (EPS) production in liquid culture of a valuable medicinal fungus Cordyceps sinensis Cs-HK1. In the medium containing 20 g l-1 glucose and 6 g l-1 peptone as the sole nitrogen source, the Cs-HK1 fungal mycelia formed smooth and spherical pellets about 1.8-mm mean diameter. The mycelial pellets became less uniform at pH (4.0-5.0) lower than the optimum (pH 6.0) or turned to Wlamentous form at higher pH (8-9). Surfactants added to the medium inhibited pellet formation, resulting in smaller and looser pellets. Tween 80 exhibited a remarkable promoting eVect on EPS production, increasing the EPS yield more than twofold at 1.5% (w/v), which was most probably attributed to the stimulation of EPS biosynthesis and release from the fungal cells by Tween 80.
Original languageEnglish
Pages (from-to)623-628
Number of pages6
JournalJournal of Industrial Microbiology and Biotechnology
Issue number4
Publication statusPublished - 1 Apr 2012


  • Cordyceps sinensis
  • Exopolysaccharide
  • Liquid culture
  • Mycelial morphology
  • Tween 80

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Medicine(all)

Cite this