Effects of surface-active medium additives on insect cell surface hydrophobicity relating to cell protection against bubble damage

Wu Jianyong, Qian Ruan, Lam H Y Peter

Research output: Journal article publicationJournal articleAcademic researchpeer-review

25 Citations (Scopus)

Abstract

A number of medium additives such as Pluronic F68, methylcellulose, and serum have been shown to decrease the adhesion of animal cells to air bubbles, thus reducing cell damage by the bubbles at rupture. The effect may be associated with the interactions between the additives and the cells. One possible mechanism is that the additives adsorb to the cell membrane through a hydrophobic interaction, resulting in decreased hydrophobicity of the cell surface. This consequently reduces cell adhesion to gas bubbles. To test this hypothesis, we measured the hydrophobicity (adhesion to hydrocarbon) of two insect cell lines in the presence of medium additives including Pluronic F68, methylcellulose, polyethylene glycol (PEG), and fetal bovine serum. All these additives except PEG caused substantial reduction in cell surface hydrophobicity which was consistent with their effect of decreasing cell adhesion to gas bubbles. In addition, significant adsorption was detected for the nonionic surfactants Pluronic and PEG to the insect cells. The findings are very helpful for elucidating the mechanisms of animal cell protection by surface-active chemicals.

Original languageEnglish
Pages (from-to)341-348
Number of pages8
JournalEnzyme and Microbial Technology
Volume21
Issue number5
DOIs
Publication statusPublished - Oct 1997

Keywords

  • Insect cells
  • Protective additives
  • Surface hydrophobicity
  • Surfactant adsorption

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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