TY - JOUR
T1 - Effects of surface-active medium additives on insect cell surface hydrophobicity relating to cell protection against bubble damage
AU - Jianyong, Wu
AU - Ruan, Qian
AU - H Y Peter, Lam
N1 - Funding Information:
We would like to thank Dr. Thomas Gong of Biosys in the U.S. for his generoush elp with our cell culture work. This researchh as been funded by an internal grant of the Hong Kong Polytechnic University and grants from the Biotechnology ResearchI nstitute and the Hong Kong University of Science and Technology.
PY - 1997/10
Y1 - 1997/10
N2 - A number of medium additives such as Pluronic F68, methylcellulose, and serum have been shown to decrease the adhesion of animal cells to air bubbles, thus reducing cell damage by the bubbles at rupture. The effect may be associated with the interactions between the additives and the cells. One possible mechanism is that the additives adsorb to the cell membrane through a hydrophobic interaction, resulting in decreased hydrophobicity of the cell surface. This consequently reduces cell adhesion to gas bubbles. To test this hypothesis, we measured the hydrophobicity (adhesion to hydrocarbon) of two insect cell lines in the presence of medium additives including Pluronic F68, methylcellulose, polyethylene glycol (PEG), and fetal bovine serum. All these additives except PEG caused substantial reduction in cell surface hydrophobicity which was consistent with their effect of decreasing cell adhesion to gas bubbles. In addition, significant adsorption was detected for the nonionic surfactants Pluronic and PEG to the insect cells. The findings are very helpful for elucidating the mechanisms of animal cell protection by surface-active chemicals.
AB - A number of medium additives such as Pluronic F68, methylcellulose, and serum have been shown to decrease the adhesion of animal cells to air bubbles, thus reducing cell damage by the bubbles at rupture. The effect may be associated with the interactions between the additives and the cells. One possible mechanism is that the additives adsorb to the cell membrane through a hydrophobic interaction, resulting in decreased hydrophobicity of the cell surface. This consequently reduces cell adhesion to gas bubbles. To test this hypothesis, we measured the hydrophobicity (adhesion to hydrocarbon) of two insect cell lines in the presence of medium additives including Pluronic F68, methylcellulose, polyethylene glycol (PEG), and fetal bovine serum. All these additives except PEG caused substantial reduction in cell surface hydrophobicity which was consistent with their effect of decreasing cell adhesion to gas bubbles. In addition, significant adsorption was detected for the nonionic surfactants Pluronic and PEG to the insect cells. The findings are very helpful for elucidating the mechanisms of animal cell protection by surface-active chemicals.
KW - Insect cells
KW - Protective additives
KW - Surface hydrophobicity
KW - Surfactant adsorption
UR - http://www.scopus.com/inward/record.url?scp=0343554788&partnerID=8YFLogxK
U2 - 10.1016/S0141-0229(97)00009-4
DO - 10.1016/S0141-0229(97)00009-4
M3 - Journal article
AN - SCOPUS:0343554788
SN - 0141-0229
VL - 21
SP - 341
EP - 348
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 5
ER -